Publications by authors named "Rebekah E Oliver"
Barley yellow dwarf viruses (BYDVs) are responsible for the disease barley yellow dwarf (BYD) and affect many cereals including oat (Avena sativa L.). Until recently, the molecular marker technology in oat has not allowed for many marker-trait association studies to determine the genetic mechanisms for tolerance.
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- - Tocochromanols are important for nutrition, plant stress response, and seed longevity, and this study focuses on their genetic variations in barley using a systems biological approach.
- - Major quantitative trait loci (QTL) affecting different forms of tocochromanols were identified across three regions in a SNP linkage map, with candidate genes characterized through comparisons with rice and sequencing in various barley genotypes.
- - The study developed gene-specific markers linked to QTL and showed that certain polymorphisms in gene promoter regions relate to gene expression levels, with increased expression observed in colder conditions, supporting methods for improving barley’s nutritional profiles.
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- Mycotoxins from Fusarium species pose a significant risk to food and feed safety in oats, making breeding for resistance an important strategy.
- A study used a population of recombinant-inbred lines to map quantitative trait loci (QTL) related to mycotoxin levels and Fusarium head blight severity, identifying a key QTL for deoxynivalenol (DON) on chromosome 17A/7C.
- Several QTL for DON and Fusarium head blight were found on multiple chromosomes, and linked SNPs may help in developing oat varieties with improved resistance to these pathological threats.
Article Synopsis
- The study addresses the challenges of creating a consensus map for the complex hexaploid oat genome (Avena sativa), including the size of the genome and scarcity of molecular markers.
- It introduces new methodologies for discovering SNPs and a novel anchoring strategy, successfully resulting in the first complete physically-anchored consensus map that includes 985 SNPs.
- The findings also highlight genetic similarities with other plants, providing tools for detailed genetic analysis and a useful framework for similar research in other complex genomes.
Thirteen common wheat "Chinese Spring" (CS)-Thinopyrum junceum addition lines and three common wheat "Fukuhokomuji"(Fuku)-Elymus rectisetus addition lines were characterized and verified as disomic additions of a Th. junceum or E. rectisetus chromosome in the wheat backgrounds by fluorescent genomic in situ hybridization.
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- Genetic markers are essential in genomics, but challenges in oat genome complexity and lack of sequence data make finding and testing them difficult; this study aimed to address these issues by generating oat expressed sequence tag (EST) data and developing a method for SNP identification.
- Researchers created a bioinformatics pipeline that processed around 1 million sequence reads, resulting in the identification of 96 in silico SNPs, with 52 found to be polymorphic in a specific oat mapping population, validating their utility through high-resolution melting (HRM) analysis.
- The study concludes that the newly developed high-throughput SNP discovery pipeline and HRM genotyping method are effective for identifying and analyzing genetic diversity in oats, offering a straightforward approach to understanding their complex poly