Publications by authors named "Rebeccah Gomberg"
Article Synopsis
- DING proteins exhibit phosphatase activity which impacts HIV-1 gene transcription by dephosphorylating key factors, specifically RNA polymerase II and NF-κB subunits.
- Experimental methods such as ELISA, northern-blot, and western-blot assays were used to show that DING proteins significantly inhibit HIV-1 infection and reduce the phosphorylation of the p65 NF-κB subunit.
- The presence of DING leads to an accumulation of hypo-phosphorylated p65 NF-κB in cells, suggesting that DING disrupts the activation and nuclear import of NF-κB, ultimately suppressing its ability to promote HIV-1 transcription.
HIV-1 gene transcription is controlled by the cooperation of viral and host factors which bind to specific DNA sequences within the viral promoter spanning the long terminal repeat (LTR). Previously we showed that the St. John's Wort DING phosphatase, p27SJ, suppresses HIV-1 gene transcription by binding to the viral protein Tat and preventing its nuclear import.
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