Quantification of Hv1-induced proton translocation by a lipid-coupled Oregon Green 488-based assay.

Passive proton translocation across membranes through proton channels is generally measured with assays that allow a qualitative detection of the H-transfer. However, if a quantitative and time-resolved analysis is required, new methods have to be developed. Here, we report on the quantification of pH changes induced by the voltage-dependent proton channel Hv1 using the commercially available pH-sensitive fluorophore Oregon Green 488-DHPE (OG488-DHPE).