Pharmacokinetic Evaluation of a DSPE-PEG2000 Micellar Formulation of Ridaforolimus in Rat.

The rapamycin analog, ridaforolimus, has demonstrated potent anti-proliferative effects in cancer treatment, and it currently is being evaluated in a range of clinical cancer studies. Ridaforolimus is an extremely lipophilic compound with limited aqueous solubility, which may benefit from formulation with polymeric micelles. Herein, we report the encapsulation of ridaforolimus in 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000) (DSPE-PEG2000) via a solvent extraction technique.

High-performance liquid chromatography assay of gnetol in rat serum and application to pre-clinical pharmacokinetic studies.

A method of analysis of gnetol (2,3',5',6-tetrahydroxy-trans-stilbene) in biological fluids is necessary for the study of its kinetics and disposition in plants. A simple high-performance liquid chromatography (HPLC) method was developed for the determination of gnetol in rat serum using a reverse-phase, isocratic system. Separation was achieved using a Phenomenex® Luna C₁₈(2) column with ultraviolet detection at 305 nm.

High-performance liquid chromatographic analysis: applications to nutraceutical content and urinary disposition of oxyresveratrol in rats.

A high-performance liquid chromatographic (HPLC) method was developed for the analysis of the stilbene, oxyresveratrol. This method involves the use of a Luna C(18) column with ultraviolet detection at 320 nm. The mobile phase consisted of acetonitrile, water and formic acid (30 : 70 : 0.