Qiagen's Investigator™ Quantiplex Kit as a predictor of STR amplification success from low-yield DNA samples.

Qiagen's Investigator™ Quantiplex kit, a total human DNA quantitation kit, has a 200-base pair internal control, fast cycling time, and scorpion molecules containing a covalently linked primer, probe, fluorophore, and quencher. The Investigator™ Quantiplex kit was evaluated to investigate a value under which complete short tandem repeat (STR) failure was consistently obtained. Buccal swabs were extracted using the Qiagen QIAamp(®) DNA Blood Mini Kit, quantified with the Investigator™ Quantiplex kit using a tested half-volume reaction, amplified with the ABI AmpFlSTR(®) Identifiler kit, separated on the 3100Avant Genetic Analyzer, and data analyzed with GeneMapper(®) ID v.

Exploring the utility of genetic markers for predicting biological age.

DNA evidence can be analyzed for genetic markers to determine phenotypes such as hair and eye color, ancestry, and even age estimation. Currently, telomere length is the only genetic biomarker that has been correlated to cell replication and replicative cell senescence--both strong indicators of tissue aging in humans. Unfortunately, while many studies have found a strong correlation between telomere length and age, many data sets show extreme variability, technical assay malfunction, inadequate evaluation of other variables that can impact telomere, altogether conflicting results, or insignificant correlations due to low sample size.