Transgenesis using nuclear transfer in goats.

Nuclear transfer (NT) using transgenic donor cells is an efficient means for generation of transgenic founder goats, especially in regard to the number of animals required to produce a transgenic founder expressing the protein of interest. Vectors can be designed for organ-specific expression and secretion of recombinant proteins within the target tissue. Furthermore, donor cells can be selected for gender, genetically modified to introduce the transgene of interest and screened for incorporation of the transgene into the genome before use in NT.

Transgene expression of green fluorescent protein and germ line transmission in cloned calves derived from in vitro-transfected somatic cells.

In vitro transfection of cultured cells combined with nuclear transfer currently is the most effective procedure to produce transgenic livestock. In the present study, bovine primary fetal fibroblasts were transfected with a green fluorescent protein (GFP)-reporter transgene and used as nuclear donor cells in oocyte reconstructions. Because cell synchronization protocols are less effective after transfection, activated oocytes may be more suitable as hosts for nuclear transfer.