Horseradish Peroxidase-Catalyzed Crosslinking of Fibrin Microthread Scaffolds.

Horseradish peroxidase (HRP) has been investigated as a catalyst to crosslink tissue-engineered hydrogels because of its mild reaction conditions and ability to modulate the mechanical properties of the matrix. Here, we report the results of the first study investigating the use of HRP to crosslink fibrin scaffolds. We examined the effect of varying HRP and hydrogen peroxide (HO) incorporation strategies on the resulting crosslink density and structural properties of fibrin in a microthread scaffold format.