Artificial neural networks in the modeling of the catalytic activity of a biosensor composed of conjugated polymers and urease.

Thin films of conjugated polymer and enzyme can be used to unravel the interaction between components in a biosensor. Using artificial neural networks (ANNs) improves data interpretability and helps construct models with great capacity for classifying and processing information. The present work used kinetic data from the catalytic activity of urease immobilized in different conjugated polymers to create ANN models using time, substrate concentration, and absorbance as input variables since the models had absorbance in a posterior instant as output value to explore the predictivity of the ANNs.

Nanocomposite Films of Silver Nanoparticles and Conjugated Copolymer in Natural and Nano-Form: Structural and Morphological Studies.

The use of conjugated polymers (CPs) and metallic nanoparticles is an interesting way to form nanocomposites with improved optical properties. For instance, a nanocomposite with high sensitivity can be produced. However, the hydrophobicity of CPs may hamper applications due to their low bioavailability and low operability in aqueous media.

Conjugated polymers as Langmuir and Langmuir-Blodgett films: Challenges and applications in nanostructured devices.

Initially developed for classic systems composed of fatty acids and phospholipids, the Langmuir and Langmuir-Blodgett (LB) techniques allow the fabrication of nanometer-scale devices at self-assembly interfaces with high control over the thickness and molecular architecture. Their application in the research and production of new plastic materials has grown considerably over the past few decades due to the efficiency of conjugated polymers (CPs) for the production of light-emitting diodes, flexible displays, solar cells, and other photoelectronic devices. The structuring of polymers at different interfaces is not trivial as this class of macromolecules can undergo through different processes of folding/unfolding, which hinders the formation of stable Langmuir monolayers and, consequently, the production of Langmuir-Blodgett films.

Langmuir and Langmuir-Blodgett Films of Poly[(9,9-dioctylfluorene)--(3-hexylthiophene)] for Immobilization of Phytase: Possible Application as a Phytic Acid Sensor.

In this work, the copolymer poly[(9,9-dioctylfluorene)--(3-hexylthiophene)] was employed as a matrix for immobilizing phytase, aiming at the detection of phytic acid. The copolymer was spread on the air-water interface forming Langmuir monolayers and phytase adsorbed from the aqueous subphase. The interactions between the copolymer and the enzyme components were investigated with surface pressure and surface potential-area isotherms, Brewster angle microscopy, and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS).