Publications by authors named "Rebeca Alonso Monge"

Candidaalbicans normally colonizes the human gastrointestinal tract as a commensal. Studying fungal factors involved in colonizing the mammalian gastrointestinal tract requires mouse models with altered microbiota. We have obtained strains of C.

View Article and Find Full Text PDF

Candida albicans is a pathobiont in humans that forms part of the mycobiota in healthy individuals and can cause different pathologies upon alterations of the host defenses. The mammalian gut is clinically relevant as this niche is the most common pool for bloodstream-derived infections. The ability of C.

View Article and Find Full Text PDF
Article Synopsis
  • * Researchers created mutants lacking Hog1 MAP kinase that overproduce Wor1, enabling these mutants to establish themselves as commensals in the mouse gut and even compete with wild-type Candida cells.
  • * The enhanced fitness of these Wor1 overproducing mutants is linked to better adherence to surfaces, increased production of enzymes like proteinase and phospholipase, and reduced filamentation in lab conditions, while showing no virulence in a systemic candidiasis model in mice.
View Article and Find Full Text PDF

is a commensal yeast that inhabits the gastrointestinal tract of humans; increased colonization of this yeast in this niche has implicated the master regulator of the white-opaque transition, Wor1, by mechanisms not completely understood. We have addressed the role that this transcription factor has on commensalism by the characterization of strains overexpressing this gene. We show that overexpression causes an alteration of the total lipid content of the fungal cell and significantly alters the composition of structural and reserve molecular species lipids as determined by lipidomic analysis.

View Article and Find Full Text PDF

The commensal and opportunistic pathogen is an important cause of fungal diseases in humans, with the gastrointestinal tract being an important reservoir for its infections. The study of the mechanisms promoting the commensal state has attracted considerable attention over the last few years, and several studies have focused on the identification of the intestinal human mycobiota and the characterization of genes involved in its establishment as a commensal. In this work, we have barcoded 114 clinical isolates to identify strains with an enhanced fitness in a murine gastrointestinal commensalism model.

View Article and Find Full Text PDF

is a commensal yeast that inhabits the gastrointestinal tract of humans. The master regulator of the white-opaque transition has been implicated in the adaptation to this commensal status. A proteomic analysis of cells overexpressing this transcription factor () suggested an altered metabolism of carbon sources and a phenotypic analysis confirmed this alteration.

View Article and Find Full Text PDF
Article Synopsis
  • The Hog1 MAPK is crucial for answering osmotic stress and also helps the pathogen deal with various other stresses, influencing its survival and ability to flourish in different environments.
  • The study reveals that Hog1 is vital for maintaining lipid balance, as mutants lacking Hog1 accumulate lipid droplets under osmotic stress, leading to cell permeability issues.
  • Cek1, another MAPK related to osmotic stress response, does not affect lipid homeostasis, suggesting that Hog1 is the primary MAP kinase managing these processes and that lipid metabolism changes make mutants more susceptible to osmotic stress.
View Article and Find Full Text PDF
Article Synopsis
  • The Mitogen-Activated Protein kinase (MAPK) pathways in fungi, particularly the HOG pathway, are crucial for responding to environmental changes and stress, impacting their survival and colonization in hosts.
  • A study found that the antifungal Micafungin (MF) had the same minimum inhibitory concentration in both a parent strain and a mutant, indicating no difference in susceptibility, and both strains showed similar impaired cell viability after treatment.
  • Unlike the positive control Amphotericin B, MF did not activate specific antioxidant genes or influence reactive oxygen species levels, suggesting that MF's toxic effects do not involve the Hog1 MAPK pathway or oxidative stress in affected cells.
View Article and Find Full Text PDF

is an important human fungal pathogen responsible for tens of millions of infections as well as hundreds of thousands of severe life-threatening infections each year. MAP kinase (MAPK) signal transduction pathways facilitate the sensing and adaptation to external stimuli and control the expression of key virulence factors such as the yeast-to-hypha transition, the biogenesis of the cell wall, and the interaction with the host. In the present study, we have combined molecular approaches and infection biology to analyse the role of MAPK pathways during an epithelial invasion.

View Article and Find Full Text PDF

The HOG MAP kinase pathway plays a crucial role in the response to different stresses in the opportunistic pathogen Candida albicans. The polyene amphotericin B (AMB) has been reported to trigger oxidative stress in several pathogenic fungi, including C. albicans.

View Article and Find Full Text PDF

displays the ability to adapt to a wide variety of environmental conditions, triggering signaling pathways and transcriptional regulation. Sko1 is a transcription factor that was previously involved in early hypoxic response, cell wall remodeling, and stress response. In the present work, the role of mutant in o and studies was explored.

View Article and Find Full Text PDF
Article Synopsis
  • CRISPR-Cas systems are powerful tools for genome manipulation, particularly the Class 2 type II system, which has been widely studied across various organisms including mammalian cells, plants, fungi, and bacteria.
  • * Pathogenic fungi are significant contributors to human diseases, making genetic manipulation crucial for developing new therapies and antifungal treatments.
  • * The review focuses on advancements in using CRISPR systems for human pathogenic fungi, highlighting improvements in research utility and exploring potential future applications.*
View Article and Find Full Text PDF

Clustered regularly interspaced short palindromic repeat (CRISPR) methodology is not only an efficient tool in gene editing but also an attractive platform to facilitate DNA, RNA, and protein interactions. We describe here the implementation of a CRISPR-based system to regulate expression in the clinically important yeast By fusing an allele of Cas9 devoid of nuclease activity to a transcriptional repressor (Nrg1) or activator (Gal4), we were able to show specific repression or activation of the tester gene , encoding the cytosolic catalase. We generated strains where a 1.

View Article and Find Full Text PDF
Article Synopsis
  • - Certain yeasts produce killer toxins (mycocins) that can harm sensitive yeast and fungi, a phenomenon observed in various environmental species.
  • - Recent research found cheese-derived yeast strains that produce these toxins and indicated that mutants lacking the MAPK Hog1 signal pathway are more susceptible to the mycocins, unlike other mutants.
  • - The study revealed that while Hog1's phosphorylation is crucial for survival against these toxins and coping with osmotic and oxidative stresses, overactive catalase does not provide protection, hinting at other stress-related mechanisms involved.
View Article and Find Full Text PDF

The transcriptional regulator Wor1 has been shown to induce the GUT transition, an environmentally triggered process that increases the fitness of in the mouse gastrointestinal tract. We have developed strains where the expression of this gene is driven from the strong and tightly regulated tetracycline promoter. These cells retain the main characteristics reported for GUT cells albeit they show defects in the initial stages of colonization.

View Article and Find Full Text PDF

Arsenic is a toxic metalloid widespread in nature. Recently, it has been demonstrated a main role of the transcription factor Pho4 in the acquisition of tolerance to arsenic-derived compounds, arsenite and arsenate in Candida albicans. Here, the effect of these compounds on this pathogenic yeast has been analyzed.

View Article and Find Full Text PDF
Article Synopsis
  • This text serves as a correction to an article appearing on page 2133 in volume 7.
  • The article referenced has the PubMed Identifier (PMID) 28111572.
  • The correction likely addresses errors or clarifications in the originally published work.
View Article and Find Full Text PDF

Eukaryotic cell cycle progression in response to environmental conditions is controlled via specific checkpoints. Signal transduction pathways mediated by MAPKs play a crucial role in sensing stress. For example, the canonical MAPKs Mkc1 (of the cell wall integrity pathway), and Hog1 (of the HOG pathway), are activated upon oxidative stress.

View Article and Find Full Text PDF

Aim: To investigate the role of Cat1 overproduction in Candida albicans.

Materials & Methods: Strains overproducing the CAT1 gene were constructed.

Results: Cells overproducing CAT1 were found to be more resistant to some oxidants and mammalian phagocytic cells.

View Article and Find Full Text PDF

The Pho4 transcription factor is required for growth under low environmental phosphate concentrations in Saccharomyces cerevisiae. A characterization of Candida albicans pho4 mutants revealed that these cells are more susceptible to both osmotic and oxidative stress and that this effect is diminished in the presence of 5% CO2 or anaerobiosis, reflecting the relevance of oxygen metabolism in the Pho4-mediated response. A pho4 mutant was as virulent as wild type strain when assayed in the Galleria mellonella infection model and was even more resistant to murine macrophages in ex vivo killing assays.

View Article and Find Full Text PDF

The cell wall integrity pathway (CWI) plays an important role in the biogenesis of the cell wall in Candida albicans and other fungi. In the present work, the C. albicans MKK2 gene that encodes the putative MAPKK of this pathway was deleted in different backgrounds and the phenotypes of the resultant mutants were characterised.

View Article and Find Full Text PDF

Arsenate (As (V)) is the dominant form of the toxic metalloid arsenic (As). Microorganisms have consequently developed mechanisms to detoxify and tolerate this kind of compounds. In the present work, we have explored the arsenate sensing and signaling mechanisms in the pathogenic fungus Candida albicans.

View Article and Find Full Text PDF

Different signal transduction pathways mediated by MAP kinases have been described in Candida albicans. These pathways sense different stimuli and, therefore, elaborate specific responses. Hog1 was identified as the MAPK that is primarily involved in stress response and virulence, while Cek1 was more specific to cell wall biogenesis, mating and biofilm formation.

View Article and Find Full Text PDF
Article Synopsis
  • * The transmembrane protein Opy2 in Candida albicans is essential for activating Cek1 phosphorylation in response to stimuli, affecting cell wall integrity and growth resumption.
  • * Mutants lacking Opy2 are more susceptible to cell wall stress and show reduced virulence in pathogenic models, while over-expressing Opy2 in related yeast helps mitigate certain environmental stresses.
View Article and Find Full Text PDF