Re-Evaluation of the Species Complex in Australia.

The species complex is highly variable morphologically and causes powdery mildew on a wide range of species, including stone fruit. A taxonomic revision of the species complex in 2020 identified 12 species, seven of which were newly characterised. In order to clarify which species of this complex are present in Australia, next generation sequencing was used to isolate the fungal ITS+28S and host chloroplast gene regions from 56 powdery mildew specimens of stone fruit and ornamental species accessioned as or sp.

Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales).

The purpose of this study was to identify a reliable DNA extraction protocol to use on 25-year-old powdery mildew specimens from the reference collection VPRI in order to produce high quality sequences suitable to address taxonomic phylogenetic questions. We tested 13 extraction protocols and two library preparation kits and found the combination of the E.Z.