Phenolic composition, antioxidant, and cytotoxic effects on HeLa and HepG2 cancer cell lines of grown in Turkey.

This study screened flavonoids and phenolic acids, antioxidant and cytotoxic effects of leaf and fruit samples. The RP-HPLC-DAD analysis allowed the identification of hesperidin, epicatechin, epigallocatechin, benzoic, -hydroxybenzoic, vanillic, protocatechuic, syringic, caffeic, ferulic, sinapic and -coumaric acids in various extracts. Fruit alkaline-hydrolysable phenolic acids extract (BHPA), leaf bound phenolic acids from basic hydrolysis-2 extract (BPBH) and leaf free flavan-3-ol extract exhibited the largest DPPH, OH and NO radicals scavenging activity, respectively.

Purification, Biochemical Characterization and Decolorization Efficiency of Laccases from Peach and Cherry Cultures of Pleutorus eryngii: A Comparative Study.

Background: Laccases (Lacs) are used potentially in industrial and biotechnological applications such as decolorization of dyes, degradation of industrial effluents, delignification, etc. thanks to their large varieties of substrate specificities and excellent catalytic efficiencies. The efficient utilizations of Lacs in these applications mostly depend on the identifying their biochemical properties.

Decolorization and degradation potential of enhanced lignocellulolytic enzymes production by Pleurotus eryngii using cherry waste from industry.

Lignocellulosic wastes accumulate in large quantities and thus cause environmental issues. Cherry waste (CW) of them collected from industry was used as the substrate to increase production of lignocellulolytic enzymes, laccase (Lac), manganese peroxidase (MnP), lignin peroxidase (LiP), carboxymethyl cellulase (CmCase), xylanase, exoglucanase, β-glucosidase (BGLA), by Pleurotus eryngii. Then, the decolorizations of some azo dyes were examined.

Peach and Cherry Agroindustrial Wastes: New and Economic Sources for the Production of Lignocellulolytic Enzymes.

Pleurotus eryngii was incubated on both peach and cherry agroindustrial wastes by solid state fermentation for 30 days without/with pretreatment conditions. The lignocellulosic substrates were pretreated with dilute acid and alkaline solutions, hot water before incubation. The maximum carboxymethyl cellulase and xylanase activities peaked on 3rd and 5th days under control conditions of both wastes, respectively.

Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization.

Lignocellulosic wastes are generally produced in huge amounts worldwide. Peach waste of these obtained from fruit juice industry was utilized as the substrate for laccase production by Pleurotus eryngii under solid state bioprocessing (SSB). Its chemical composition was determined and this bioprocess was carried out under stationary conditions at 28 °C.

Optimization of cultural conditions for biosurfactant production by Pleurotus djamor in solid state fermentation.

Being eco-friendly, less toxic, more biodegradable and biocompatible, biological surfactants have higher activity and stability compared to synthetic ones. In spite of the fact that there are abundant benefits of biosurfactants over the synthetic congeners, the problem related with the economical and large scale production proceeds. The utilization of several industrial wastes in the production media as substrates reduces the production cost.

Ammonium nitrate and iron nutrition effects on some nitrogen assimilation enzymes and metabolites in Spirulina platensis.

The effect of various concentrations of ammonium nitrate (5-60 mM), an economical nitrogen source, on the growth, nitrate-ammonium uptake rates, production of some pigments and metabolites, and some nitrogen assimilation enzymes such as nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), and glutamate synthase (GOGAT) in Spirulina platensis (Gamont) Geitler was investigated. Ten millimolars of ammonium nitrate stimulated the growth, production of pigments and the other metabolites, and enzyme activities, whereas 30 and 60 mM ammonium nitrate caused inhibition. In the presence of 10 mM ammonium nitrate, different concentrations of iron were tried in the growth media of S.

Concurrent biosurfactant and ligninolytic enzyme production by Pleurotus spp. in solid-state fermentation.

Pleurotus spp. is known as one of the significant producers of ligninolytic enzymes which efficiently degrade lignocellulosic materials. Recent studies on potential of biosurfactant production by Pleurotus spp.

Extracellular ligninolytic enzymes production by Pleurotus eryngii on agroindustrial wastes.

Pleurotus eryngii (DC.) Gillet (MCC58) was investigated for its ligninolytic ability to produce laccase (Lac), manganese peroxidase (MnP), aryl alcohol oxidase (AAO), and lignin peroxidase (LiP) enzymes through solid-state fermentation using apricot and pomegranate agroindustrial wastes. The reducing sugar, protein, lignin, and cellulose levels in these were studied.

Production of ligninolytic enzymes by solid-state fermentation using Pleurotus eryngii.

Pleurotus eryngii (DC.) Gillet (MCC58) was investigated for its ability to produce various ligninolytic enzymes such as laccase (Lac), manganese peroxidase (MnP), aryl alcohol oxidase (AAO), and lignin peroxidase (LiP) by solid-state fermentation (SSF), which was carried out using a support substrate from the fruit juice industry. The chemical content of grape waste from this industry was studied.

Manganese peroxidase production by immobilized Phanerochaete chrysosporium BKM-F-1767 in a cell bioreactor.

Manganese-dependent peroxidase (MnP) production was performed in an immobilized cell bioreactor in which Phanerochaete chrysosporium BKM-F-1767 was immobilized on polystyrene foam. The immobilized cell culture yielded significantly greater MnP activity than the conventional stationary liquid culture. Cultivation was carried out in batch mode; the effect of glucose concentration was investigated and growth kinetics parameters were found as, micromax=0.

In vitro antioxidant properties of Cucurbita pepo L. male and female flowers extracts.

Total antioxidant capacities, 2,2-diphenyl-1-picrylhydrazyl (DPPH.), hydroxyl (HO.), scavenging activities, and total phenolic values were determined in extracts of Cucurbita pepo L.

A novel carrier for Phanerochaete chrysosporium immobilization.

Phanerochaete chrysosporium BKMF-1767 cells were immobilized on different carriers. The optimum carrier according to adsorbed P. chrysosporium cells number (86.