Elucidating the Secondary Metabolite Biosynthesis Networks in Underutilized Tree Bean (Parkia timoriana) Through Integrated Metabolomic and Transcriptomic Approaches.

The tree bean (Parkia timoriana (DC). Merr) is an underutilized legume and is abundantly found in Southeast Asia. It is valued for its nutritious pods and cultivated for food and timber.

The Root-Tuber Trypsin Inhibitor of Winged Bean and Its Anti-cancerous Activity Against Osteosarcoma Cell-Line.

Trypsin inhibitor from the root-tuber of underutilized legume Winged bean (Psophocarpus tetragonolobus (L.) DC.) (WbT-TI) was purified using ion exchange chromatography followed by size-exclusion chromatography.

β Pore-forming Protein-based Evolutionary Divergence of from .

Introduction: The first vertebrates were jawless fish, or , whose evolution diverged into jawed fish, or , around 550 million years ago.

Methods: In this study, we investigated β PFT proteins' evolutionary divergence of lamprey immune protein from , reportedly possessing anti-cancer activity, into Dln1 protein from . Both proteins showed structural and functional divergence, and shared evolutionary origin.

Recombinant expression and preliminary characterization of Peptidyl-prolyl cis/trans-isomerase Rrd1 from Saccharomyces cerevisiae.

Sacchromycescerevisiae Peptidyl-prolylcis/trans-isomerase Rrd1 has been linked to DNA repair, bud morphogenesis, advancement of the G1 phase, DNA replication stress, microtubule dynamics and is also necessary for the quick decrease in Sgs1p levels in response to rapamycin. In present study, Rrd1 gene was amplified by standard PCR and subsequently cloned downstream to bacteriophage T7 inducible promoter and lac operator of expression vector pET21d(+). Additionally, immobilized metal affinity chromatography (IMAC) was used to purify the protein upto its homogeneity, and its homogeneous purity was further confirmed through western blotting.

Genome-wide identification and expression analysis of the HD2 protein family and its response to drought and salt stress in species.

Histone deacetylase 2 (HD2) proteins play an important role in the regulation of gene expression. This helps with the growth and development of plants and also plays a crucial role in responses to biotic and abiotic stress es. HD2s comprise a CH-type Zn finger at their C-terminal and an HD2 label, deacetylation and phosphorylation sites, and NLS motifs at their N-terminal.

Reducing the biosynthesis of condensed tannin in winged bean ( (L.) DC.) by virus-induced gene silencing of anthocyanidin synthase (ANS) gene.

Unlabelled: The Underutilized legume-winged bean ( (L.) DC.) and its various parts are infested with condensed tannin (CT) or proanthocyanidin (PA).

Synthesis of an unusual quinazoline alkaloid: theoretical and experimental investigations of its structural, electronic, molecular and biological properties.

An unusual quinazoline alkaloid (1) was obtained when 2-aminobenzaldehyde was refluxed with pyrrolidine in ethanol for 12 h. The synthesized compound was characterized using spectral data analysis augmented with X-ray and literature precedent. Single crystal analysis depicted four conformations differing slightly in bond angles and bond lengths.