Membrane Remodeling and Matrix Dispersal Intermediates During Mammalian Acrosomal Exocytosis.

To become fertilization-competent, mammalian sperm must undergo a complex series of biochemical and morphological changes in the female reproductive tract. These changes, collectively called capacitation, culminate in the exocytosis of the acrosome, a large vesicle overlying the nucleus. Acrosomal exocytosis is not an all-or-nothing event but rather a regulated process in which vesicle cargo disperses gradually.

In-cell structures of conserved supramolecular protein arrays at the mitochondria-cytoskeleton interface in mammalian sperm.

Mitochondria-cytoskeleton interactions modulate cellular physiology by regulating mitochondrial transport, positioning, and immobilization. However, there is very little structural information defining mitochondria-cytoskeleton interfaces in any cell type. Here, we use cryofocused ion beam milling-enabled cryoelectron tomography to image mammalian sperm, where mitochondria wrap around the flagellar cytoskeleton.

Synthesis of low-cost bentonite/Duranta erecta's fruit powder imbedded alginate beads and its application in surfactant removal.

High industrialization and improved medical facilities are deteriorating aquatic bodies through untreated effluents. This study is aimed to design and characterize the bentonite, Duranta erecta, and their hybrid-alginate beads for the removal of cetyltrimethylammonium bromide (CTAB) from its aqueous solution. D.

The multi-scale architecture of mammalian sperm flagella and implications for ciliary motility.

Motile cilia are molecular machines used by a myriad of eukaryotic cells to swim through fluid environments. However, available molecular structures represent only a handful of cell types, limiting our understanding of how cilia are modified to support motility in diverse media. Here, we use cryo-focused ion beam milling-enabled cryo-electron tomography to image sperm flagella from three mammalian species.

Knowledge and awareness on novel coronavirus spread among dental fraternities in Visakhapatnam, India: A questionnaire-based survey.

Introduction: The pandemic caused by novel coronavirus (severe acute respiratory syndrome coronavirus 2) in Wuhan, China, in December 2019 is a highly contagious disease. The World Health Organization has declared the outbreak of coronavirus diseases (COVID-19) as a global public health emergency. Currently, the research on novel coronavirus is still in the primary stage.

Effect of different obturating techniques and sealers on the removal of filling materials during endodontic retreatment.

Background: Adequate removal of previous root filling materials is an important objective during endodontic retreatment.

Aim: This study was aimed to evaluate the influence of obturation quality and sealer type on the removal of root filling materials during endodontic retreatment using micro-computed tomography (μ-CT).

Materials And Methods: Ninety extracted mandibular premolars were randomly assigned to two groups ( = 45) based on the type of sealer (AH Plus and BioRoot RCS) and three sub-groups based on the obturating technique used, i.

Lockdown of colleges and universities due to COVID-19: Any impact on the educational system in India?

Pneumonia triggered by novel corona virus (SARS-CoV-2) in Wuhan, China, in December 2019 is an extremely contagious disease. The WHO has stated the outbreak of COVID-19 (corona virus) as a pandemic issue. Corona virus cases started increasing day by day in India, also from the time the first case was diagnosed on January 30, 2020.

Looking back and looking forward: contributions of electron microscopy to the structural cell biology of gametes and fertilization.

Mammalian gametes-the sperm and the egg-represent opposite extremes of cellular organization and scale. Studying the ultrastructure of gametes is crucial to understanding their interactions, and how to manipulate them in order to either encourage or prevent their union. Here, we survey the prominent electron microscopy (EM) techniques, with an emphasis on considerations for applying them to study mammalian gametes.

Effect of intratooth location and thermomechanical cycling on microtensile bond strength of bulk-fill composite resin.

Context: The initial bond strength and potential durability of an adhesive restoration are significantly affected by regional variations in dentin composition.

Aim: The aim of this study is to evaluate the influence of dentin location and thermomechanical cyclic loading on microtensile bond strength of bulk-fill composite resin to coronal dentin.

Materials And Methods: Disto-occlusal cavity preparations were done on 60 extracted human mandibular molars with pulpal floor depth of 1.

Microhardness and Penetration of Artificial White Spot Lesions Treated with Resin or Colloidal Silica Infiltration.

Introduction: Infiltration of early enamel lesions by materials having remineralizing capacity seems to improve aesthetics and arrests caries progression.

Aim: To evaluate and compare the surface microhardness and penetration depth of a low viscosity resin and colloidal silica nanoparticle infiltrates into artificially created white spot lesions.

Materials And Methods: Forty extracted human central incisors were embedded in acrylic resin blocks exposing the labial surfaces of the crowns.

Exchange transfusion: a low-cost alternative for severe childhood Guillain-Barré syndrome.

The high cost and nonavailability of plasmapheresis and intravenous immunoglobulin are prohibitive for the treatment of Guillain-Barré syndrome in resource-poor settings. Exchange transfusion can be an alternative therapy for severe disease in children. The effectiveness of exchange transfusion was evaluated in nine children (median age 6 years) with severe Guillain-Barré syndrome (functional disability score >/= 4).