Optimization of keratinase production and enzyme activity using response surface methodology with Streptomyces sp7.

A two-step response surface methodology (RSM) study was conducted for the optimization of keratinase production and enzyme activity from poultry feather by Streptomyces sp7. Initially different combinations of salts were screened for maximal production of keratinase at a constant pH of 6.5 and feather meal concentration of 5 g/L.

Purification and characterization of an alkaline keratinase from Streptomyces sp.

A protease producing bacterial culture ('S7') was isolated from slaughterhouse waste samples, Hyderabad, India. It was related to Streptomyces sp. on the basis of biochemical properties and 16S rRNA gene sequencing.