Publications by authors named "Ratsimandresy Holinirina Dina Miora"
The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the center position, yielding to the violation of energy conservation.
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- Image reconstruction in fluorescence microscopy relies heavily on the accuracy of the point spread function (PSF) of the optical system used.
- A MATLAB toolbox was previously developed to calculate realistic vector Fourier-based PSF, which takes into account various aberrations.
- This study validates these numerical methods experimentally, demonstrating a high accuracy (over 0.97) in fitting simulated results to actual data and achieving a contrast level of up to 95%.