A New Serotyping Method of Streptococcus pneumoniae Based on CRISPR/Cas9-Targeted Sequencing.

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) application for targeted sequencing has made a breakthrough in the genomic research era. High diversity in the capsular polysaccharide (cps) locus of Streptococcus pneumoniae has hampered identification of the serotype. This study developed a new serotyping method for S.

Pneumococcal transposon profiling associated with macrolide, tetracycline, and chloramphenicol resistance from carriage isolates of serotype 19F in Indonesia.

Genetic evolution of resistance due to mutations and transposon insertions is the primary cause of antimicrobial resistance in Streptococcus pneumoniae. Resistance to macrolide, tetracycline, and chloramphenicol is caused by the insertion of specific genes that carried by transposon (Tn). This study aims to analyze transposon profiling associated with macrolide, tetracycline, and chloramphenicol resistance from carriage isolates of S.

A narrative review of genomic characteristics, serotype, immunogenicity, and vaccine development of capsular polysaccharide.

This narrative review describes genomic characteristic, serotyping, immunogenicity, and vaccine development of capsular polysaccharide (CPS). CPS is a primary virulence factor of . The genomic characteristics of CPS, including the role of biosynthetic gene and genetic variation within (capsule polysaccharide) locus which may lead to serotype replacement are still being investigated.

Whole genome sequencing data of isolated from Indonesian population.

is the leading cause of bacterial pneumonia, bacteremia, and meningitis. Indonesia introduced the pneumococcal conjugate vaccine (PCV) nationwide in 2022. In this study, we present whole genome sequence (WGS) data of 94 isolates that were obtained from hospitalized patients, healthy children, and adult groups from different regions prior to PCV program in Indonesia.