Bactericidal Effect of Triple Antibiotic Paste against Enterococcus faecalis in Dentinal Tubules: An Ex Vivo Study.

Objective: The aim of this study was to investigate the bactericidal effect of various concentrations of triple antibiotic paste (TAP) against Enterococcus faecalis (E. faecalis) in dentinal tubules using a bacterial culture assay and confocal laser scanning microscope (CLSM).

Methods: Ninety human teeth were contaminated with E.

Effect of brushing on surface roughness, fluoride release, and biofilm formation with different tooth-colored materials.

Background/purpose: Tooth brushing, material mechanical ageing procedure, is the most effective way in removing biofilm. The purpose of this study was to investigate the surface roughness, fluoride-release, and S. mutans biofilm formation on various tooth-colored restorative materials before and after brushing.

Buffering capacity and antibacterial properties among bioactive glass-containing orthodontic adhesives.

This study was to evaluate the acid-buffering capacity and antibacterial properties of orthodontic adhesives containing bioactive glasses (BAGs) (45S5, 45S5F, S53P4), Hydroxyapatite, beta-tricalcium phosphate, and Canasite. Fillers comprising 15 wt% bioactive glasses, HAp, β-TCP, and Canasite incorporated with 55 wt% silanated glass were added to a mixture of UDMA/TEGDMA. Acid-buffering capacity was tested by exposing disc-shaped samples of each adhesive to medium of bacteria-produced acids, and pH changes were recorded at 24 and 48 h.

Physicochemical and osteogenic properties of chairside processed tooth derived bone substitute and bone graft materials.

To analyze physicochemical such as surface structures, the crystallinity, chemical composition, calcium phosphate dissolution and osteogenic properties of tooth derived bone substitute (TDBS) processed chair-side and other grafting materials. The number of anaerobic and facultative anaerobic bacteria in the supernatant of processed TDBS was determined. Human osteoblasts were co-cultured with TDBS or allograft in transwell system to examine cell migration.

evaluation of local antibiotic delivery via fibrin hydrogel.

Background/purpose: Fibrin hydrogel is commonly used as hemostatic agent and scaffold but it is questionable for carrying antibiotics. Thus, this study aimed to investigate whether the fibrin hydrogel can be used to deliver the optimal concentration of ciprofloxacin against oral pathogen.

Materials And Methods: The optimal concentration of ciprofloxacin was investigated from broth microdilution technique against three common oral bacteria.

Bacterial leakage and marginal adaptation of various bioceramics as apical plug in open apex model.

Aim: The aim of the present study was to investigate bacterial leakage and marginal adaptation of bioceramic apical plugs.

Methods: Extracted human mandibular premolars were prepared to simulate open apex using No. 4 Peeso reamer in retrograde direction.

The efficiency of topical anesthetics as antimicrobial agents: A review of use in dentistry.

Topical anesthetics are commonly used in oral & maxillofacial surgery to control pain in the oral cavity mucosa before local anesthetic injection. These anesthetic agents come in many forms, developed for different usages, to minimize adverse reactions, and for optimal anesthetic efficiency. Earlier studies have revealed that these agents may also limit the growth of microorganisms in the area of anesthetic application.

Bacterial leakage and micro-computed tomography evaluation in round-shaped canals obturated with bioceramic cone and sealer using matched single cone technique.

Objectives: To evaluate sealing ability of root canals obturated with bioceramic-impregnated gutta percha cone (BCC) or gutta percha (GP), with bioceramic sealer (BCS) or AH Plus (AH; Dentsply-Maillefer), in roundly-prepared canals using matched single-cone technique, based on bacterial leakage test, and to analyze obturation quality using micro-computed tomography (CT) analysis.

Materials And Methods: Ninety-two distobuccal roots of maxillary molars were prepared using nickel-titanium files to apical size 40/0.06.

Induction of inducible nitric oxide synthase (iNOS) in Porphyromonas gingivalis LPS-treated mouse macrophage cell line (RAW264.7) requires Toll-like receptor 9.

Objective: The aim of this study is to investigate the involvement of TLR9 in the regulation of iNOS expression and nitric oxide (NO) production in Porphyromonas gingivalis LPS-treated mouse macrophages.

Methods: Mouse macrophage cell line (RAW264.7) was transfected with siRNAs against TLR9 and then stimulated with P.

EFFECTS OF SHORT-TERM USE OF XYLITOL CHEWING GUM AND MOLTITOL ORAL SPRAY ON SALIVARY STREPTOCOCCUS MUTANS AND ORAL PLAQUE.

The purpose of this study was to investigate the short-term effects of xylitol chewing gum and maltitol spray on the concentration of salivary mutans streptococci (MS) and on the plaque index. Eighty-one second, third and fourth year dental and dental assistant students with a salivary MS concentration > 103 CFU/ml cultured on mitis salivarius bacitracin (MSB) agar were included in the study. The age range of subjects was 18-23 years.

Hypochlorite solution for root canal irrigation that lacks a chlorinated odor.

Objectives: This is an study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. The antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (NaOCl) solutions.

Materials And Methods: Trichloroisocyanuric acid (TCA) was used as the source of hypochlorite ions in solution.

Antimicrobial effect of topical local anesthetic spray on oral microflora.

Background: To evaluate the antimicrobial activity of lidocaine (LD) topical anesthetic spray against oral microflora.

Methods: Antimicrobial effects of 10% LD spray were assessed against six bacterial cultures obtained from volunteers: , , , , , and . The filter papers contained 50-µl LD, brain heart infusion (BHI) broth, or 0.

EFFECTIVENESS OF AN ORAL HYGIENE EDUCATION PROGRAM COMBINED WITH FLUORIDE MOUTHRINSE AMONG VISUALLY IMPAIRED STUDENTS IN BANGKOK, THAILAND.

The objective of this study was to assess the effectiveness of oral hygiene education kits (OHE kits) and 0.05% sodium fluoride mouth rinse among visually impaired students (VIS) in Bangkok, Thailand. Seventy-five VIS aged 10-12 years were included in the study and examined for plaque index (PI), gingival index (GI) and mutans streptococci (MS) salivary levels at baseline and after intervention.

Variation of expression defects in cell surface 190-kDa protein antigen of Streptococcus mutans.

Streptococcus mutans, which consists of four serotypes, c, e, f, and k, possesses a 190-kDa cell surface protein antigen (PA) for initial tooth adhesion. We used Western blot analysis to determine PA expression in 750 S. mutans isolates from 150 subjects and found a significantly higher prevalence of the isolates with PA expression defects in serotypes f and k compared to serotypes c and e.

Multilocus sequence typing analysis of Streptococcus mutans strains with the cnm gene encoding collagen-binding adhesin.

Streptococcus mutans is one of the oral pathogens associated with infective endocarditis (IE). With respect to bacterial binding ability to the extracellular matrix, the Cnm protein, a cell surface collagen-binding adhesin of S. mutans, is known as one of the possible virulence factors with regard to IE.

Translocation of Porphyromonas gingivalis infected monocytes and associated cellular responses.

Porphyromonas gingivalis (P. gingivalis), an important periodontal pathogen in adult chronic periodontitis, has been reported to colocalize in human atheromatous lesions. We have studied the phagocytosis and survival of P.

Modulation of gamma interferon-induced major histocompatibility complex class II gene expression by Porphyromonas gingivalis membrane vesicles.

Gamma interferon (IFN-gamma)-induced endothelial cells actively participate in initiating immune responses by interacting with CD4(+) T cells via class II major histocompatibility complex (MHC) surface glycoproteins. Previously, Porphyromonas gingivalis membrane vesicles were shown to selectively inhibit IFN-gamma-induced surface expression of HLA-DR molecules by human umbilical cord vascular endothelial cells. In this study, we demonstrated an absence of HLA-DR alpha mRNA from IFN-gamma-induced cells in the presence of P.