sp. nov. and sp. nov., two novel actinobacteria isolated from mangrove sediments and leaves of halophyte in Thailand.

A polyphasic approach was used to characterize two novel actinobacterial strains, designated PKS22-38 and LSe1-13, which were isolated from mangrove soils and leaves of halophyte (L.), respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that they belonged to the genus and were most closely related to three validly published species with similarities ranging from 98.

Streptomyces acidicola sp. nov., isolated from a peat swamp forest in Thailand.

A novel actinobacterium, designated strain K1PN6, was isolated from soil sample collected in Kantulee peat swamp forest, Surat Thani province, Thailand. The morphological, chemotaxonomic, and phylogenetic characteristics were consistent with its classification in the genus Streptomyces. Based on 16S rRNA gene sequence analysis, strain K1PN6 showed highest similarity to Streptomyces phyllanthi PA1-07 (98.

Amycolatopsis pithecelloba sp. nov., a novel actinomycete isolated from roots of Pithecellobium dulce in Thailand.

An actinomycete strain RM579 isolated from roots of Pithecellobium dulce in Thailand was studied using the polyphasic taxonomic approach. The results of comparative 16S rRNA gene sequence and phylogenetic analysis showed that strain RM579 belonged to the family Pseudonocardiaceae and it was most closely related to members of the genera Amycolatopsis (≤94.5% sequence similarity) and Haloechinothrix (≤93.

Cryptosporangium eucalypti sp. nov., an actinomycete isolated from Eucalyptus camaldulensis roots.

A novel actinomycete, designated strain EURKPP3H10T, was isolated from surface-sterilized roots of Eucalyptus camaldulensis Dehnh., collected from Kamphaengphet Silvicultural Research Station, Kamphaengphet province, Thailand. The taxonomic position of strain EURKPP3H10T was studied using a polyphasic approach.

Three novel species of the genus Kibdelosporangium; Kibdelosporangium kanagawaense sp. nov., Kibdelosporangiumrhizosphaerae sp. nov. and Kibdelosporangium rhizovicinum sp. nov.

The taxonomic positions of three actinomycete isolates, K08-0175T, K10-0543T and K12-0791T, which were isolated from plant root and rhizospheric soil samples were subjected to a polyphasic taxonomic study. On the basis of the results of phylogenetic analysis and morphological and chemotaxonomic characteristics, these three strains were classified as representing members of the genus Kibdelosporangium. These strains were observed to produce both long chains of rod-shaped spores and sporangium-like structures with well-defined walls on aerial hyphae.

1-Methoxypyrrole-2-carboxamide-A new pyrrole compound isolated from Streptomyces griseocarneus SWW368.

A new pyrrole compound, 1-methoxypyrrole-2-carboxamide, was obtained from a culture broth of Streptomyces griseocarneus SWW368, which was isolated from the rhizospheric soil under a Para rubber tree (Hevea brasiliensis). The chemical structure was elucidated by 1D NMR, 2D NMR, and MS, as a pyrrole ring with a N-methoxy group and a primary amide group. It exhibited antibacterial properties against Kocuria rhizophila, Staphylococcus aureus and Xanthomonas campestris pv.

Nonomuraea purpurea sp. nov., an actinomycete isolated from mangrove sediment.

A polyphasic approach was used to verify the novel actinomycete, strain 1SM4-01T, isolated from mangrove sediment collected from Ranong Province, Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism was a member of the genus Nonomuraea and was most closely related to Nonomuraea syzygii GKU 164T (98.7 % sequence similarity), Nonomuraea rhizophila YIM 67092T (98.

Nocardiopsissediminis sp. nov., isolated from mangrove sediment.

A filamentous actinomycete, designated strain 1SS5-02T, was isolated from mangrove sediment collected from Ranong province, Thailand. The strain formed aerial and substrate mycelia composed of long, branched hyphae. Aerial mycelia differentiated into non-motile, rod-shaped spores.

Actinorhabdospora filicis gen. nov., sp. nov., a new member of the family Micromonosporaceae.

The actinomycete strains K12-0408T and K12-0792 were isolated on CM-cellulose agar from rhizosphere soil of a pteridophytic plant collected in Tokyo prefecture, Japan. Their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains K12-0408T and K12-0792 were positioned within the family Micromonosporaceae.

Proposal of Sphaerimonospora cavernae gen. nov., sp. nov. and transfer of Microbispora mesophila (Zhang et al., 1998) to Sphaerimonospora mesophila comb. nov. and Microbispora thailandensis (Duangmal et al., 2012) to Sphaerimonospora thailandensis comb. nov.

The actinomycete strain N74T, isolated from cave soil, was studied using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain N74T formed a stable, distinct lineage cluster together with Microbispora thailandensis NN276T (99.3% similarity) and Microbispora mesophila JCM 3151T (97.

Actinopolyspora salinaria sp. nov., a halophilic actinomycete isolated from solar saltern soil.

The taxonomic position of the halophilic actinobacterial strain, HS05-03T, isolated from solar saltern soil, was determined using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence of the strain showed that it formed a distinct evolutionary lineage in the genus Actinopolyspora. The organism was most closely related to the type strains of the species Actinopolyspora xinjiangensis (98.

sp. nov., isolated from mangrove sediment.

An aerobic, Gram-stain-positive, motile, coccus-shaped actinobacterium, designated strain L2-1-L1, was isolated from mangrove sediment in Thailand. The organism was deep orange, oxidase-negative and catalase-positive. Growth occurred at temperatures between 17 and 32 °C and with NaCl concentrations up to 10 %.

Streptomyces oryzae sp. nov., an endophytic actinomycete isolated from stems of rice plant.

An actinomycete strain S16-07(T), isolated from surface-sterilized stems of rice plant (Oryza sativa L.), was characterized using a polyphasic approach. Phylogenetic analysis of 16S rRNA gene sequences indicated affiliation of the strain belonged to the genus Streptomyces.

Sphaerisporangium rufum sp. nov., an endophytic actinomycete from roots of Oryza sativa L.

An endophytic actinomycete, strain R10-82(T), isolated from surface-sterilized roots of rice (Oryza sativa L.) was studied using a polyphasic approach. Strain R10-82(T) produced branching substrate mycelia and developed spherical spore vesicles on aerial hyphae containing non-motile spores.

Isolation of rhizospheric and roots endophytic actinomycetes from Leguminosae plant and their activities to inhibit soybean pathogen, Xanthomonas campestris pv. glycine.

In this study, actinomycetes from roots and rhizospheric soils of leguminous plants were isolated using starch casein agar supplemented with antifungal and antibacterial antibiotics. Three hundred and seventeen actinomycetes were isolated with 77 isolates obtained from plant roots and 240 isolates from rhizospheric soils. Analysis of whole-organism hydrolysates showed that 289 strains were rich in the LL-isomer of diaminopimelic acid, a result consistent with their assignment to the streptomycetes.

Microbispora thailandensis sp. nov., an actinomycete isolated from cave soil.

The taxonomic position of actinomycete strain NN276(T), isolated from cave soil, was studied using the polyphasic taxonomic approach. A phylogenetic tree based on 16S ribosomal RNA (rRNA) gene sequences showed that the isolate formed a distinct evolutionary linage with the genus Microbispora, with M. mesophila JCM 3151(T) as its closest phylogenetic neighbor (97.

Sphaerisporangium siamense sp. nov., an actinomycete isolated from rubber-tree rhizospheric soil.

A Gram-positive aerobic actinomycete, designated SR14.14(T), isolated from the rhizospheric soil of rubber tree was determined taxonomically using a polyphasic approach. The organism contained meso-diaminopimelic acid and the N-acetyl type of peptidoglycan.

Amycolatopsis samaneae sp. nov., isolated from roots of Samanea saman (Jacq.) Merr.

A novel actinomycete, designated strain RM287(T), was isolated from surface-sterilized roots of Samanea saman (Jacq.) Merr., collected from Bangkok, Thailand.

Saccharopolyspora phatthalungensis sp. nov., isolated from rhizosphere soil of Hevea brasiliensis.

The taxonomic position of a rhizosphere soil isolate, designated strain SR8.15T, was determined by using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-separated sub-branch within the radiation encompassing the genus Saccharopolyspora.