Detection of plasmid-mediated quinolone resistance in clinical isolates of strains in Hamadan, West of Iran.

Plasmid mediated quinolone resistance (PMQR) determinants have arisen as a significant concern in recent years. The aim of this study was screening of resistant-clinical isolates to fluoroquinolone antibiotics and detection of and genes. For this purpose we collected 100 fluoroquinolone-resistant which were from 3 hospitals in Hamadan, west provinces of Iran, between October 2012 and June 2013.

Identification of Group B Streptococci Using 16S rRNA, cfb, scpB, and atr Genes in Pregnant Women by PCR.

Streptococcus agalactiae is acommensalorganism, but it may cause infection in susceptible hosts. The aim of this study was to evaluate PCR assay compared with conventional culture method for direct detection of Streptococcus agalactiae. Total of 203 paired low vaginal swabs were collected from women at 35-37 weeks of pregnancy from June 2013 through February 2014 for detection of Streptococcus agalactiae using PCR assay targeting 16S rRNA, cfb, scpB, and atr genes and culture method following broth enrichment.

Prevalence of Enterotoxin Genes and Antibacterial Susceptibility Pattern of Staphylococcus aureus Strains Isolated from Animal Originated Foods in West of Iran.

Objectives: The aims of our study were to evaluate the prevalence of Staphylococcus aureus (S. aureus) strains in food samples of animal origin, examine their antibacterial susceptibility pattern, and to detect staphylococcal enterotoxin (SEs) genes and the mecA gene in isolated S. aureus strains using the polymerase chain reaction (PCR).

Effect of apically separated rotary instruments on bacterial leakage of obturated root canals using resilon.

The purpose of this study was to determine the effect of a separated rotary instrument on the time required for bacterial penetration of obturated root canals using Resilon. Eighty-four extracted human maxillary premolars with single canals were used in this study. Group 1 consisted of teeth obturated with gutta-percha and AH-26 sealer (n=15); Group 2 consisted of teeth obturated similarly to Group 1 but without a separated file; Group 3 consisted of teeth obturated with Resilon and Epiphany sealer (n=15); and Group 4 consisted of teeth obturated similarly to Group 3 but without a separated file.

Antimicrobial effect of three new and two established root canal irrigation solutions.

This study sought to assess the efficacy of two established and three relatively new root canal irrigants against five different microorganisms using the agar diffusion method. Sodium hypochloride (NaOCl)-based irrigants demonstrated the most effective antimicrobial activity.

Bacterial microleakage and post space timing for two endodontic sealers: an in vitro study.

Aim: The effects of immediate versus delayed post space preparation on the apical seal using resin and zinc oxide eugenol (ZOE) sealers were compared by a bacterial leakage model.

Methodology: Eighty-six premolars were randomly assigned to four experimental groups of 20 teeth. Three teeth were assigned to each control group, either positive (filled only with guttapercha) or negative (not obturated but root surfaces completely covered).

Urinary tract infections: bacteriology and antibiotic resistance patterns.

The aim of this study was to identify the bacteria causing community acquired urinary tract infections (UTI) and detection of antibiotics resistance of isolates in 912 children below 18 years in the west of Iran. Data were analyzed for 4 age groups: infants, toddlers, preteens and teens. Fourteen antibiotics were tested by gel-diffusion method.

Diagnostic multiplex polymerase chain reaction assay for the identification of Pseudomonas aeruginosa from the skin biopsy specimens in burn wound infections and detection of antibiotic susceptibility.

Objective: To identify Pseudomonas aeruginosa (P. aeruginosa) from the skin biopsy specimens in burn wound infections by multiplex polymerase chain reaction (M-PCR) and detection of antimicrobial susceptibility of isolates from culture.

Methods: We conducted this cross-sectional study in 140 patients with wound infections who admitted to the referral burn center of Motahari, Tehran, Iran, during a 12-month period from 2005-2006.