Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4T cells of healthy individuals of Indian origin.

Human induced pluripotent stem cell-lines (iPSCs) of Indian origin NCCSi005A and NCCSi006A were established by reprogramming of CD4T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of two healthy female donors. Reprogramming was achieved using integration free, Sendai viral vector system expressing cocktail of transcription factors KOS, hc-MYC and hKLF4. Both the established cell-lines showed alkaline phosphatase activity, expressed stemness markers, exhibited normal female karyotype and displayed potential for tri-lineage differentiation.