Biofilm-specific determinants of enterococci pathogen.

Amongst all Enterococcus spp., E. faecalis and E.

A sustainable synthesis of polyhydroxyalkanoate from stubble waste as a carbon source using MTCC 2475.

Polyhydroxyalkanoates (PHAs) are biodegradable polymers that can be produced from lignocellulosic biomass by microorganisms. Cheap and readily available raw material, such as corn stover waste, has the potential to lessen the cost of PHA synthesis. In this research study, corn stover is pretreated with NaOH under conditions optimized for high cellulose and low lignin with central composite design (CCD) followed by characterization using Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), and scanning electron microscopy (SEM).

Acid hydrolysis of periderm for accumulation of polyhydroxyalkanoates in MTCC 2475.

Polyhydroxyalkanoates are a class of biodegradable, biocompatible polymers composed of polyesters of R-hydroxyalkanoic acids and deposited intracellularly by a variety of microorganisms which have potential to serve as alternative to commercial plastic. Bioplastics are gaining attention due to sustainability, biodegradability, biocompatibility, and lower carbon footprint. Nevertheless, the commercialization of PHA is predominantly hindered by the elevated production expenses arising primarily from the use of a pure sugar substrate.

Conversion of Corn Stover for Microbial Enzymes Production by Phanerochaete chrysosporium.

Phanerochaete chrysosporium, a white rot fungus, exhibits remarkable capabilities in producing various extracellular enzymes. These microbial enzymes find extensive applications in disrupting the intricate structure of plant cell walls, decolorizing synthetic dyes, and facilitating pulp extraction, among other functions. The process of solid-state fermentation stands out as an economical and sustainable approach, ideal for achieving high yields in enzyme production using lignocellulosic biomass as a substrate.

Potato Peel Waste as an Economic Feedstock for PHA Production by Bacillus circulans.

Polymers of hydroxy alkanoates (PHA), also known as biodegradable, biocompatible plastic, are potential alternatives to petrochemical-based plastics. PHA is synthesized by microbes in their cytoplasm in the form of inclusion bodies in stress conditions such as nitrogen, oxygen, and phosphorus with excessive amounts of carbon. Sugar extracted from potato peel in the form of hydrolysate was employed as a carbon source for PHA production after acidic hydrolysis.

Potential of Rhodosporidium toruloides for Fatty Acids Production Using Lignocellulose Biomass.

Microbial lipids are ideal for developing liquid biofuels because of their sustainability and no dependence on food crops. Especially the bioprocess for microbial lipids may be made economical by using sustainable approaches, e.g.

Biofilm patterns in gram-positive and gram-negative bacteria.

The Gram-positive and Gram-negative bacteria are attributable to matrix-enclosed aggregates known as biofilms. Biofilms are root cause of industrial biofouling and characterized by antimicrobial resistance during infections. Many biofilm studies examine specific Gram type cultures, whereas nearly all biofilm communities in nature comprise both Gram-negative and Gram-positive bacteria.

Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains.

This study investigated the potential of grass biomass as a feedstock for mcl-PHA production. Pretreatments (2% NaOH at 120°C or hot water at 120°C) of perennial ryegrass were employed alone or in combination with sodium chlorite/acetic acid (SC/AA) delignification to evaluate the enzymatic digestibility and subsequent utilization of resultant sugars by Pseudomonas strains. NaOH pretreated sample had better digestibility than raw and hot water treated samples and this hydrolysate supported good growth of all tested strains with limited mcl-PHA (6-17% of cell dry mass (CDM)) accumulation.

Trends in bacterial trehalose metabolism and significant nodes of metabolic pathway in the direction of trehalose accumulation.

The current knowledge of trehalose biosynthesis under stress conditions is incomplete and needs further research. Since trehalose finds industrial and pharmaceutical applications, enhanced accumulation of trehalose in bacteria seems advantageous for commercial production. Moreover, physiological role of trehalose is a key to generate stress resistant bacteria by metabolic engineering.

Saccharification of Kans grass using enzyme mixture from Trichoderma reesei for bioethanol production.

Bioethanol is one of the alternatives of the conventional fossil fuel. In present study, effect of different carbon sources on the production of cellulolytic enzyme (CMCase) from Trichoderma reesei at different temperatures, duration and pH were investigated and conditions were optimized. Acid treated Kans grass (Saccharum sponteneum) was subjected to enzymatic hydrolysis to produce fermentable sugars which was then fermented to bioethanol using Saccharomyces cerevisiae.