Ultrasonic atomizer based development of pH sensor for real time analysis.

Fluorescent pH biosensors have gained importance owing to their low cost utilization in real time monitoring of biological and food samples in comparison to conventional pH meters. The research reports a novel method of ultrasonic atomization for developing a fluorescent pH sensor for real-time analysis made of Fluorescein isothiocyanate (FITC)-dextran/FITC-dextran-Tris (2, 2'-bipyridyl) dichlororuthenium (II) hexahydrate as indicator and reference fluorophores, respectively. The process of ultrasonic atomization ensures formation of monodisperse dye immobilized alginate microspheres ensuring efficient pH sensing.

Fluorescent Biocompatible Platinum-Porphyrin-Doped Polymeric Hybrid Particles for Oxygen and Glucose Biosensing.

Near infrared (NIR) fluorophores like Pt-porphyrin along with analyte specific enzymes require co-encapsulation in biocompatible and biodegradable carriers in order to be transformed into implantable biosensors for efficient and continuous monitoring of analytes in patients. The main objective of this research is to develop natural, biodegradable, biocompatible and a novel co-encapsulated system of Pt-porphyrin encapsulated polymeric nanoparticle and nano-micro hybrid carriers. A sequential emulsification-solvent evaporation and an air driven atomization technique was used for developing above matrices and testing them for fluorescence based oxygen and glucose biosensing.

pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors.

Kidney diseases remain often undiagnosed due to inefficient screening methods available at patient's disposal. Early diagnosis and effective management of kidney problems can best be addressed by the development of biosensors for commonly occurring clinical biomarkers. Here we report the development of single fluorophore and dual fluorophore ratiometric biosensors based on alginate microspheres for pH and urea analysis in urine samples.

Multifunctional alginate microspheres for biosensing, drug delivery and magnetic resonance imaging.

This research aims to develop and investigate a multifunctional implantable system capable of biosensing, drug delivery and magnetic resonance imaging (MRI) for continuous monitoring, controlled anti-inflammatory drug delivery and imaging, respectively. A glucose biosensor, diclofenac sodium (Diclo) and magnetic nanoparticles (MNP) were used as the biosensor component, anti-inflammatory agent and MRI contrast agent, respectively. MNP were synthesized by the co-precipitation technique and loaded with the sensor and drug components into alginate microspheres using a commercial droplet generator.