Characterization of a novel subfamily 1.4 lipase from Bacillus licheniformis IBRL-CHS2: Cloning and expression optimization.

This study focuses on a novel lipase from Bacillus licheniformis IBRL-CHS2. The lipase gene was cloned into the pGEM-T Easy vector, and its sequences were registered in GenBank (KU984433 and AOT80658). It was identified as a member of the bacterial lipase subfamily 1.

Bioconversion of Used Transformer Oil into Polyhydroxyalkanoates by sp. Strain AAAID-1.5.

In this research, the utilisation of used transformer oil (UTO) as carbon feedstock for the production of polyhydroxyalkanoate (PHA) was targeted; with a view to reducing the environmental challenges associated with the disposal of the used oil and provision of an alternative to non-biodegradable synthetic plastic. sp. strain AAAID-1.

Characterization of sp. Ab3 Lipase and Its Coexpression with LEA Peptides.

sp. is a yellowish Gram-negative bacterium that is usually characterized by high concentrations of sphingophospholipids as lipid components. As microbial enzymes have been in high demand in industrial fields in the past few decades, this study hopes to provide significant information on lipase activities of sp.

Bioprospecting and Molecular Identification of Used Transformer Oil-Degrading Bacteria for Bioplastics Production.

One of the major impediments to the commercialization of biodegradable plastic is the high cost of substrate. Consequently, there is a continuous search for effective microorganisms and cheaper carbon substrates to reduce the high production cost. In this study, waste transformer oil-degrading bacteria were isolated from soil, wastewater, and sediment samples, using a mineral salt medium (MSM) supplemented with 1% waste transformer oil as the sole carbon source.

Psychrophilic Lipase from Arctic Bacterium.

A lipase producer psychrophilic microorganism isolated from Arctic sample was studied. The genomic DNA of the isolate was extracted using modified CTAB method. Identification of the isolate by morphological and 16S rRNA sequence analysis revealed that the isolate is closely related to (97% similarity).

Cloning and Expression of a Subfamily 1.4 Lipase from IBRL-CHS2.

We report on the cloning of the lipase gene from IBRL-CHS2 and the expression of the recombinant lipase. DNA sequencing analysis of the cloned lipase gene showed that it shares 99% identity with the lipase gene from ATCC 14580 and belongs to subfamily 1.4 of true lipases based on amino acid sequence alignment of various lipases.

Purification and Characterization of a Thermostable Lipase from Geobacillus thermodenitrificans IBRL-nra.

Thermostable lipase from Geobacillus thermodenitrificans IBRL-nra was purified and characterized. The production of thermostable lipase from Geobacillus thermodenitrificans IBRL-nra was carried out in a shake-flask system at 65°C in cultivation medium containing; glucose 1.0% (w/v); yeast extract 1.

Hell's Gate globin I: an acid and thermostable bacterial hemoglobin resembling mammalian neuroglobin.

Hell's Gate globin I (HGbI), a heme-containing protein structurally homologous to mammalian neuroglobins, has been identified from an acidophilic and thermophilic obligate methanotroph, Methylacidiphilum infernorum. HGbI has very high affinity for O(2) and shows barely detectable autoxidation in the pH range of 5.2-8.