Label-free detection and profiling of individual solution-phase molecules.

Most chemistry and biology occurs in solution, in which conformational dynamics and complexation underlie behaviour and function. Single-molecule techniques are uniquely suited to resolving molecular diversity and new label-free approaches are reshaping the power of single-molecule measurements. A label-free single-molecule method capable of revealing details of molecular conformation in solution would allow a new microscopic perspective of unprecedented detail.

A 2D chiral microcavity based on apparent circular dichroism.

Engineering asymmetric transmission between left-handed and right-handed circularly polarized light in planar Fabry-Pérot (FP) microcavities would enable a variety of chiral light-matter phenomena, with applications in spintronics, polaritonics, and chiral lasing. Such symmetry breaking, however, generally requires Faraday rotators or nanofabricated polarization-preserving mirrors. We present a simple solution requiring no nanofabrication to induce asymmetric transmission in FP microcavities, preserving low mode volumes by embedding organic thin films exhibiting apparent circular dichroism (ACD); an optical phenomenon based on 2D chirality.

Label-free observation of individual solution phase molecules.

The vast majority of chemistry and biology occurs in solution, and new label-free analytical techniques that can help resolve solution-phase complexity at the single-molecule level can provide new microscopic perspectives of unprecedented detail. Here, we use the increased light-molecule interactions in high-finesse fiber Fabry-Pérot microcavities to detect individual biomolecules as small as 1.2 kDa with signal-to-noise ratios >100, even as the molecules are freely diffusing in solution.

ECOG and BMI as preoperative risk factors for severe postoperative complications in ovarian cancer patients: results of a prospective study (RISC-GYN-trial).

Background: Accompanying co-morbidities in patients with ovarian cancer are of major relevance for scheduling debulking surgery, especially in the anesthesiological consultations. Aim of this study was to evaluate the impact of co-morbidities and patient characteristics on postoperative complications.

Methods: Patients undergoing maximal cytoreductive surgery were prospectively enrolled from October 2015 to January 2017.

Effects of sarcopenia and malnutrition on morbidity and mortality in gynecologic cancer surgery: results of a prospective study.

Background: Malnutrition and sarcopenia often occur simultaneously in cancer patients and are thought to have harmful effects on both surgical and oncological outcomes. Therefore, we want to evaluate the effects of sarcopenia and malnutrition on severe postoperative complications and overall survival in gynecologic cancer patients.

Methods: We assessed nutritional parameters and run a bioelectrical impedance analysis in 226 women.

Inclusive groups can avoid the tragedy of the commons.

The public goods game is a famous example illustrating the tragedy of the commons (Hardin in Science 162:1243-1248, 1968). In this game cooperating individuals contribute to a pool, which in turn is distributed to all members of the group, including defectors who reap the same rewards as cooperators without having made a contribution before. The question is now, how to incentivize group members to all cooperate as it maximizes the common good.

Role of predictive markers for severe postoperative complications in gynecological cancer surgery: a prospective study (RISC-Gyn Trial).

Background: Surgery for gynecological cancer involves highly invasive and complex procedures potentially associated with various complications, which can cause extended hospital stays and delay of subsequent therapy, with a detrimental effect on the prognosis. The aim of this study was to explore and define the predictors of severe postoperative complications in patients undergoing surgery for gynecologic cancer.

Methods: Patients undergoing surgery for gynecologic cancers were analyzed prospectively from October 2015 through January 2017.

A Signal Adaptive Prediction Filter for Video Coding Using Directional Total Variation: Mathematical Framework and Parameter Selection.

In this paper we combine video compression and modern image processing methods. Iterative filter methods for prediction signals based on classic inpainting methods are introduced and extensive parameter tests are described. In order to construct an alternative prediction filter for video coding, techniques originally employed for inpainting are applied.

Peptidyl-Prolyl-/-Isomerases Mip and PpiB of Contribute to Surface Translocation, Growth at Suboptimal Temperature, and Infection.

The gammaproteobacterium is the causative agent of Legionnaires' disease, an atypical pneumonia that manifests itself with severe lung damage. , a common inhabitant of freshwater environments, replicates in free-living amoebae and persists in biofilms in natural and man-made water systems. Its environmental versatility is reflected in its ability to survive and grow within a broad temperature range as well as its capability to colonize and infect a wide range of hosts, including protozoa and humans.

Evaluation of a novel ELISA for the tumor-associated antigen CA 72-4 in patients with ovarian cancer.

Aim: Cancer antigen 72-4 (CA 72-4) is an established tumor marker in ovarian cancer. We evaluated a new solid-phase ELISA (DRG TM-CA 72-4 ELISA).

Materials & Methods: Repeated measures of test samples and controls were performed to evaluate reliability and reproducibility.

Legionella-protozoa-nematode interactions in aquatic biofilms and influence of Mip on Caenorhabditis elegans colonization.

Legionella pneumophila, the causative agent of Legionnaireś disease, is naturally found in aquatic habitats. The intracellular life cycle within protozoa pre-adapted the "accidental" human pathogen to also infect human professional phagocytes like alveolar macrophages. Previous studies employing the model organism Caenorhabditis elegans suggest that also nematodes might serve as a natural host for L.

Novel Cycloheximide Derivatives Targeting the Moonlighting Protein Mip Exhibit Specific Antimicrobial Activity Against Legionella pneumophila.

Macrophage infectivity potentiator (Mip) and Mip-like proteins are virulence factors in a wide range of pathogens including Legionella pneumophila. These proteins belong to the FK506 binding protein (FKBP) family of peptidyl-prolyl-cis/trans-isomerases (PPIases). In L.

Peptidylprolyl cis-trans isomerases of Legionella pneumophila: virulence, moonlighting and novel therapeutic targets.

Legionella pneumophila, typically a parasite of free-living protozoa, can also replicate in human alveolar macrophages and lung epithelial cells causing Legionnaires' disease in humans, a severe atypical pneumonia. The pathogen encodes six peptidylprolyl cis-trans isomerases (PPIases), which generally accelerate folding of prolyl peptide bonds, and influence protein folding. PPIases can be divided into three classes, cyclophilins, parvulins and FK506-binding proteins (FKBPs).

Human lung tissue explants reveal novel interactions during Legionella pneumophila infections.

Histological and clinical investigations describe late stages of Legionnaires' disease but cannot characterize early events of human infection. Cellular or rodent infection models lack the complexity of tissue or have nonhuman backgrounds. Therefore, we developed and applied a novel model for Legionella pneumophila infection comprising living human lung tissue.

microRNA and the pathogenesis of ovarian cancer--a new horizon for molecular diagnostics and treatment?

Ovarian cancer is the leading cause of death among gynecologic malignancies and despite advances in treatment, more than 50% of all patients will experience recurrence, resulting in worse overall prognosis. Therefore, identification of novel biomarkers for ovarian cancer is of significant interest. microRNA (miRNA) constitute a class of small gene regulatory RNA molecules (18-24 nt) and by sequence complementarity, they negatively regulate messenger RNA (mRNA) translation of target genes.

An optimized in vitro blood-brain barrier model reveals bidirectional transmigration of African trypanosome strains.

The transmigration of African trypanosomes across the human blood-brain barrier (BBB) is the critical step during the course of human African trypanosomiasis. The parasites Trypanosoma brucei gambiense and T. b.

1T-TiSe2: semimetal or semiconductor?

Even though the semimetallic behavior of 1T-TiSe2 seemed to be well established by band structure calculations and photoemission results, this conclusion has been challenged recently. Two high-resolution photoemission investigations deduced semiconducting behavior, however with a very small band gap. Such conclusion from photoemission is afflicted, in principle, by the problem of determining an unoccupied conduction band by photoemission.

Master's swimming: an example of successful aging in competitive sport.

Master's swimming has been one of the most successful master's athletic organizations over the past few decades. It creates an excellent environment for older athletes to stay fit and develop friendships. Exercise has been shown to be important in keeping an aging population healthy by reducing risk factors associated with chronic diseases.

[Low urinary tract infection and cystoscopic findings in Urogynecology Service in the Instituto Nacional de Perinatología].

Introduction: The pathogenesis of urinary tract infection and related to the host and pathogens. There are three associated factors: incontinence, cystocele and residual urine.

Objectives: To know the prevalence of urinary tract infections and uropathogens in urogyneacologic patients of the Instituto Nacional de Perinatología describe the cystoscopic findings in patients with positive urocultures and antibiogram.

Investigation of annexin V binding to lymphocytes after extracorporeal photoimmunotherapy as an early marker of apoptosis.

Background: Induction of programmed cell death is assumed to be a possible effect of extracorporeal photoimmunotherapy (ECPI).

Objective: In the present study lymphocytes of patients with cutaneous T cell lymphoma undergoing ECPI were investigated for early apoptotic events.

Methods: Annexin V, known for its selective affinity to phospholipids, was used to detect early phases of apoptosis.