Phytochemical Composition and Antimicrobial and Antibiofilm Effect of Hydroethanolic Extract against and .

and are opportunistic pathogens, and both are involved in different oral infections. This work aimed to analyze the phytochemical composition of hydroethanolic extract and to evaluate its antimicrobial and antibiofilm action against (ATCC 6538) and (ATCC 19606; multi-resistant clinical strains 58004, 50098, 566006, and H557). hydroethanolic extract was prepared, and the content of soluble solids, flavonoids, and phenols was quantified.

Synergistic effect of the verapamil and amphotericin B against Cryptococcus neoformans.

Cryptococcus neoformans is an encapsulated yeast that can cause cryptococcosis and cryptococcal meningitis, which conventional treatment involves antifungal drugs such as polyenes, flucytosine, azoles, and their combinations. However, the high cost, toxicity, and increase in fungi resistance to antifungal agents stimulate the search for therapeutic strategies such as drug repurposing and combination therapy. This study evaluated the activity of the antihypertensive verapamil (VEH) alone and combined with amphotericin B (AmB) against C.

Synergistic Antibiofilm Action of and Brazilian Green Propolis Hydroethanolic Extracts against Multidrug-Resistant Strains of and and Their Biocompatibility on Human Keratinocytes.

The accumulated dental biofilm can be a source of oral bacteria that are aspirated into the lower respiratory tract causing ventilator-associated pneumonia in hospitalized patients. The aim of this study was to evaluate the synergistic antibiofilm action of the produced and phytochemically characterized extracts of and Brazilian green propolis (BGP) hydroethanolic extracts against multidrug-resistant clinical strains of and , in addition to their biocompatibility on human keratinocyte cell lines (HaCaT). For this, High-performance liquid chromatography analysis of the plant extracts was performed; then the minimum inhibitory and minimum bactericidal concentrations of the extracts were determined; and antibiofilm activity was evaluated with MTT assay to prevent biofilm formation and to reduce the mature biofilms.

In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformans.

Cryptococcus neoformans is a yeast that mainly affects immunocompromised individuals and causes meningoencephalitis depending on the immune status of the host. The present study aimed to validate the efficacy of selective serotonin reuptake inhibitors, fluoxetine hydrochloride (FLH) and paroxetine hydrochloride (PAH), alone and in combination with amphotericin B (AmB) against C. neoformans.

Antifungal and anti-biofilm effect of the calcium channel blocker verapamil on non-albicans Candida species.

Candida is a human fungal pathogen that causes a wide range of diseases. Candida albicans is the main etiologic agent in these diseases; however, infections can be caused by non-albicans Candida species. Virulence factors such as biofilm production, which protect the fungus from host immunity and anti-fungal drugs, are important for the infection.

Recent Advances in the Use of Model to Study Immune Responses against Human Pathogens.

The use of invertebrates for in vivo studies in microbiology is well established in the scientific community. Larvae of are a widely used model for studying pathogenesis, the efficacy of new antimicrobial compounds, and immune responses. The immune system of larvae is structurally and functionally similar to the innate immune response of mammals, which makes this model suitable for such studies.