Publications by authors named "Rao Ribeiro"
Objective: To investigate the effects of quercetin (QU), hesperetin (HT), and taxifolin (TX) on human dental pulp cells (hDPCs) chronically exposed to lipopolysaccharide (LPS).
Methods: First, the cytotoxicity (alamarBlue) and bioactivity (biomineralization, Alizarin Red) of QU, HT, and TX concentrations were evaluated on healthy hDPCs. Then, the effects of non-cytotoxic and bioactive concentrations were investigated on hDPCs after previous stimulation with E.
Objective: This study investigated the bioactive effects of retinoic acid and ascorbic acid on hSCAPs in vitro.
Design: Cells were obtained from human third molars (n=4) and characterized for mesenchymal stem cell markers by flow cytometry. The experimental groups: control (α-MEM); vehicle control group (α-MEM + 0.
Objectives: To investigate the transdentinal effects of surface reaction-type pre-reacted glass-ionomer (S-PRG) fillers on odontoblast-like cells.
Methods: An eluate of S-PRG fillers was obtained by dissolving the particles in distilled water (1:1 m/v). Dentin discs with similar permeability were mounted into artificial pulp chambers and MDPC-23 cells were seeded on their pulpal surface.
This study investigated the incorporation of sources of calcium, phosphate, or both into electrospun scaffolds and evaluated their bioactivity on human dental pulp cells (HDPCs). Additionally, scaffolds incorporated with calcium hydroxide (CH) were characterized for degradation, calcium release, and odontogenic differentiation by HDPCs. Polycaprolactone (PCL) was electrospun with or without 0.
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- The study aimed to determine if using a polymeric primer containing titanium tetrafluoride improves the effectiveness of a bleaching gel on tooth enamel, while also protecting enamel's structure and reducing toxicity.
- Standardized enamel/dentin discs were treated with varying concentrations of titanium tetrafluoride before applying a 35% bleaching gel, and several tests were conducted to measure bleaching efficacy, enamel hardness, and cytotoxic effects.
- Results showed that while the primer significantly improved bleaching outcomes (with group 10TiF showing the best results), it also helped to limit damage to the enamel's microstructure compared to the untreated control.
Aim: This study evaluated the transdentinal cytotoxic effects of enzymatic agents (EA) for chemomechanical carious tissue removal on human dental pulp cells.
Methodology: The groups were based on the performed dentine treatments (n = 8): G1: Positive Control (PC - no treatment); G2: Negative Control (NC - 35% H O for 2 min); G3: Brix 3000™ (BX) for 30 s; G4: BX for 2 min; G5: Papacarie Duo™ (PD) for 30 s; G6: PD for 2 min. The cells were evaluated for viability (VB; MTT assay) and production of reactive oxygen species (ROS; DCFH-DA assay) and nitric oxide (NO; Griess reagent).
Objectives: To investigate the response of pulp cells to the application of silver diamine fluoride (SDF) and potassium iodide (KI) on demineralized dentin.
Materials And Methods: The occlusal surfaces of human dentin discs (0.4 mm thick) with similar permeability were subjected to an artificial caries protocol, and then the discs were adapted into artificial pulp chambers.
Chronic exposure to lipopolysaccharides as an in vitro model to simulate the impaired odontogenic potential of dental pulp cells under pulpitis conditions.
J Appl Oral Sci
July 2023
Article Synopsis
- Researchers aimed to understand how chronic exposure to lipopolysaccharides (LPS) affects human dental pulp cells (HDPCs) and their ability to regenerate tissue under pulpitis conditions.
- They conducted experiments by exposing HDPCs to various concentrations of LPS, analyzing cellular responses such as gene expression, inflammation markers, and cell viability over time.
- Results showed that higher LPS concentrations led to inflammation, reduced gene expression related to tooth development, and decreased cell viability and mineralization, indicating a negative impact on the cells' regenerative potential.
Objective: To assess the potential influence of violet LED (V-LED) application time on the esthetic efficacy and cytotoxicity of a 35% HO bleaching gel.
Methodology: Stained and standardized enamel/dentin discs were subjected to one in-office tooth bleaching session (45 min), and the gel was either irradiated or not with V-LED. Thus, the following groups were established (n = 8): G1: No treatment (negative control, NC); G2: 35% HO (positive control, PC); G3: 35%HO + V-LED/15 min; G4: 35%HO + V-LED/30 min; G5: 35%HO + V-LED/45 min.
Objective: This study aimed to evaluate the esthetic efficacy, cytotoxicity, and kinetics of decomposition of hydrogen peroxide (H2O2) present in a commercial bleaching gel with 35% H2O2 (BG35%) chemically activated with manganese oxide (MnO2).
Methods And Materials: After incorporating 2 mg/mL, 6 mg/mL, and 10 mg/mL of MnO2 into BG35%, the stability of pH and temperature of the products were analyzed. To assess the esthetic efficacy (ΔE and ΔWI), the BG35%s with MnO2 were applied for 45 minutes on enamel/dentin discs (DiE/D).
Objectives: Targeting a tissue engineering-based vital pulp therapy (VPT), this study investigated the incorporation of nano-hydroxyapatite (nHA) into polycaprolactone (PCL) nanofibers, and the metabolism of human dental pulp cells (HDPCs) seeded on the scaffolds.
Methods: PCL-based solutions (10% w/v) containing nHA (0 - control; 0.5; 1.