E-Syt1 Regulates Neuronal Activity-Dependent Endoplasmic Reticulum-Plasma Membrane Junctions and Surface Expression of AMPA Receptors.

Endoplasmic reticulum (ER)-plasma membrane (PM) contact sites/junctions play important roles in cell physiology including signal transduction, ion and lipid transfer, and membrane dynamics. However, little is known about the dynamic regulation and functional roles of ER-PM junctions in neurons. Using a split green fluorescent protein-based membrane contact probe, we find that the density of ER-PM contact sites changes dynamically in the dendrites of hippocampal neurons undergoing long-term synaptic potentiation (LTP).

Comprehensive analysis of the lysine acetylome in reveals cross-talk between lysine acetylation and succinylation in LuxS.

Lysine acetylation and succinylation are both prevalent protein post-translational modifications (PTMs) in bacteria species, whereas the effect of the cross-talk between both PTMs on bacterial biological function remains largely unknown. Our previously study found lysine succinylated sites on proteins play important role on metabolic pathways in fish pathogenic . A total of 3189 lysine-acetylation sites were further identified on 1013 proteins of this pathogen using LC-MS/MS in this study.

Influence of floc size and structure on membrane fouling in coagulation-ultrafiltration hybrid process--the role of Al13 species.

Coagulation application prior to ultrafiltration process was carried out to increase humic acid (HA) removal and membrane permeability. The [Al(13)O(4)(OH)(24)(H(2)O)(12)](7+) polycation (Al(13) species) was used in the coagulant process and polyaluminum chloride (PACl) was also used for comparison. Characteristics of aggregates pre-coagulated by Al(13) species and PACl were investigated using a laser diffraction particle sizing device.