Vps4a Mediates a Unified Membrane Repair Machinery to Attenuate Ischemia/Reperfusion Injury.

Background: Cardiac ischemia/reperfusion disrupts plasma membrane integrity and induces various types of programmed cell death. The ESCRT (endosomal sorting complex required for transport) proteins, particularly AAA-ATPase Vps4a (vacuolar protein sorting 4a), play an essential role in the surveillance of membrane integrity. However, the role of ESCRT proteins in the context of cardiac injury remains unclear.

Essential role of Alix in regulating cardiomyocyte exosome biogenesis under physiological and stress conditions.

Background: Exosomes released by cardiomyocytes are essential mediators of intercellular communications within the heart, and various exosomal proteins and miRNAs are associated with cardiovascular diseases. However, whether the endosomal sorting complex required for transport (ESCRT) and its key component Alix is required for exosome biogenesis within cardiomyocyte remains poorly understood.

Methods: Super-resolution imaging was performed to investigate the subcellular location of Alix and multivesicular body (MVB) in primary cardiomyocytes.

Fault Diagnosis of Rolling Bearing Based on HPSO Algorithm Optimized CNN-LSTM Neural Network.

The quality of rolling bearings is vital for the working state and rotation accuracy of the shaft. Timely and accurately acquiring bearing status and early fault diagnosis can effectively prevent losses, making it highly practical. To improve the accuracy of bearing fault diagnosis, this paper proposes a CNN-LSTM bearing fault diagnosis model optimized by hybrid particle swarm optimization (HPSO).

Molecular characteristics of norovirus in sporadic and outbreak cases of acute gastroenteritis and in sewage in Sichuan, China.

Background: Norovirus is highly diverse and constant surveillance is essential for the prevention and control of norovirus gastroenteritis.

Methods: From 2015 to 2019, fecal samples were collected from sporadic cases and outbreaks of acute gastroenteritis reported to Sichuan center for disease control and prevention. Sewage samples were collected from a wastewater treatment plant in Sichuan.

Microfluidic Chip with Two-Stage Isothermal Amplification Method for Highly Sensitive Parallel Detection of SARS-CoV-2 and Measles Virus.

A two-stage isothermal amplification method, which consists of a first-stage basic recombinase polymerase amplification (RPA) and a second-stage fluorescence loop-mediated isothermal amplification (LAMP), as well as a microfluidic-chip-based portable system, were developed in this study; these enabled parallel detection of multiplex targets in real time in around one hour, with high sensitivity and specificity, without cross-contamination. The consumption of the sample and the reagent was 2.1 μL and 10.

Beware of pharyngeal Fusobacterium nucleatum in COVID-19.

Background: Fusobacterium nucleatum (F. n) is an important opportunistic pathogen causing oral and gastrointestinal disease. Faecalibacterium prausnitzii (F.

Metal-Organic Gel Derived N-Doped Granular Carbon: Remarkable Toluene Uptake and Rapid Regeneration.

Porous carbon materials with chemical and thermal stability and high porosity have been widely used for volatile organic compound (VOC) purification. Designing granular carbon with remarkable adsorption capacity and rapid regeneration is of great significance for the capture of VOCs from high humidity air. Herein, a series of N-doped granular carbons were synthesized by direct carbonization of metal-organic gel (MOG).

Detection of SARS-CoV-2 in fecal samples with different pretreatment methods and PCR kits.

Background: Gastrointestinal symptoms are common in COVID-19 patients and SARS-CoV-2 RNA has been detected in the patients' feces, which could lead to fecal-oral transmission. Therefore, fecal sample testing with real-time RT-PCR is highly recommended as a routine test for SARS-CoV-2 infection. However, varying rates of detection in fecal sample have been reported.

Epidemiology of norovirus gastroenteritis in hospitalized children under five years old in western China, 2015-2019.

Objectives: Norovirus is associated with one-fifth of all gastroenteritis cases, but basic epidemiological data is lacking, especially in developing countries. As long-term surveillance on norovirus gastroenteritis is scarce in western China, this study aims to update the epidemiological knowledge of norovirus gastroenteritis and to characterize the genotypes of norovirus strains.

Methods: Stool samples were collected from hospitalized children under 5 years old with gastroenteritis in Chengdu, China.

Macroporous MnO-based aerogel crosslinked with cellulose nanofibers for efficient ozone removal under humid condition.

Atmospheric ozone pollution receives worldwide concerns, and it is a big challenge to search for the practical ozone-decomposition catalyst with good moisture resistance. Herein, a light-weight and high-porosity MnO-based hybrid aerogel was synthesized with cellulose nanofibers using a facile ice-template approach, followed by freeze-drying. In the three-dimensional framework, the cellulose nanofibers serve as the skeletons to disperse MnO particles, improving the exposure of active sites on MnO.

Reflection on lower rates of COVID-19 in children: Does childhood immunizations offer unexpected protection?

The incidence of COVID-19 in children and teenagers is only about 2% in China. Children had mild symptoms and hardly infected other children or adults. It is worth considering that children are the most vulnerable to respiratory pathogens, but fatal SARS-like virus had not caused severe cases among them.

In Situ Preparation of Amphibious ZnO Quantum Dots with Blue Fluorescence Based on Hyperbranched Polymers and their Application in Bio-Imaging.

A new strategy for preparing amphibious ZnO quantum dots (QDs) with blue fluorescence within hyper-branched poly(ethylenimine)s (HPEI) was proposed in this paper. By changing [Zn]/[OH] molar ratio and heating time, ZnO QDs with a quantum yields (QY) of 30% in ethanol were obtained. Benefiting from the amphibious property of HPEI, the ZnO/HPEI nanocomposites in ethanol could be dissolved in chloroform and water, acquiring a QY of 53%, chloroform and 11% in water.

The impact of IFNL3 genotype on interferon treatment outcome in patients chronically infected with hepatitis B virus: A meta-analysis.

Background: Polymorphisms near the interferon lambda 3 (IFNL3, also known as IL28B) have been proposed to be associated with interferon (IFN)-induced hepatitis C virus (HCV) clearance, but the impact of IFNL3 variations on the result of IFN-based therapy in chronic hepatitis B (CHB) infection is still poor understood.

Methods: The purpose of this study was to evaluate the relationship between the IFNL3 polymorphisms and the effectiveness of IFN therapy in patients infected with CHB by means of meta-analysis. PubMed and Embase were utilized to identify relevant studies.

Isolation and structural elucidation of novel antimicrobial compounds from maggots of Chrysomyis megacephala Fabricius.

The excretions/secretions from the maggot of Chrysomyis megacephala Fabricius are traditionally used to treat serious infections in China. In this study, bioassay-guided fractionation led to the isolation of three novel antibacterial compounds (1-3), including important fluorinated compounds (3 and 5), together with other nine known compounds from 70% methanol extract of C. megacephala.

[Hemagglutinin gene of influenza A (H3N2) virus from human samples in the 2009 influenza epidemic in Chengdu].

Objective: To study the antigenic and genetic characteristics of influenza A (H3N2) virus in the 2009 influenza epidemics in Chengdu.

Methods: The influenza virus strains were isolated with MDCK cells from 4869 samples taken from the sentinel surveillance in 2009 in Chengdu. Hemagglutination inhibition (HI) and RT-PCR reaction tests were performed to guide the extraction of viral RNA from the culture fluid of the influenza A (H3N2) virus.