Publications by authors named "Ranjeet R Kumar"

This study examined the impact of steam-infusion on defatted peanut (Pn), chickpea (Cp), and rice (R) protein isolates (PI) and their blends. Steam infusion significantly increased protein content (up to 83.13 % in PnPI, 75.

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Yellow mosaic disease (YMD) is a major constraint for the low productivity of mungbean, mainly in South Asia. Addressing this issue requires a comprehensive approach, integrating field and challenge inoculation evaluations to identify effective solutions. In this study, an infectious clone of Begomovirus vignaradiataindiaense (MYMIV) was developed to obtain a pure culture of the virus and to confirm resistance in mungbean plants exhibiting resistance under natural field conditions.

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Pigeon pea, a protein-rich legume with low protein digestibility (PD) due to its high polyphenol content and other antinutritional factors (ANFs). Consequently, processing methods are crucial to improve PD. We investigated the effects of thermal treatments (cooking, hydrothermal, autoclaving, infrared rays) treatments and germination on modulation of PD, its properties and association with ANFs in two distinct genotypes based on polyphenol content: high (Pusa Arhar 2018-4) and low (ICP-1452).

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Developing early maturing lentil has the potential to minimize yield losses, mainly during terminal drought. Whole-genome resequencing (WGRS) based QTL-seq identified the loci governing earliness in lentil. The genetic analysis for maturity duration provided a good fit to 3:1 segregation (F), indicating earliness as a recessive trait.

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Article Synopsis
  • The study identifies a large number of insertion/deletion (InDel) polymorphisms in the lentil genome, which can help in mapping traits related to growth duration, specifically earliness in flowering.
  • Through whole-genome resequencing of two extreme bulks (early and late flowering) and a parental genotype, researchers discovered 734,716 novel InDels, with a significant amount providing potential for marker-assisted selection (MAS).
  • The identified InDel markers, especially I-SP-356.6, show promise for facilitating the transfer of early maturity traits to other lentil varieties, highlighting their value in developing faster-growing cultivars.
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  • Calcium-dependent protein kinase (CDPK) is crucial for plant signaling, acting as a thermo-sensor, and researchers identified 28 full-length putative CDPKs in wheat.
  • The study focused on the TaCPK-1 gene, which showed the highest expression in thermotolerant wheat leaves under heat stress, indicating its role in thermotolerance.
  • Analysis revealed that TaCPK-1 correlates with other stress-related genes and displayed increased calcium levels, suggesting its importance in signaling during heat stress in wheat.
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Unlabelled: an elite heat-stress tolerant shrub, grows in arid regions of desert. However, its molecular mechanism responsible for heat stress tolerance is unexplored. Therefore, we analysed whole transcriptome of Jaisalmer (heat tolerant) and Godhra (heat sensitive) genotypes of to understand its molecular mechanism responsible for heat stress tolerance.

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Quantitative trait loci (QTL) mapping is used for the precise localization of genomic regions regulating various traits in plants. Two major QTLs regulating Soil Plant Analysis Development (SPAD) value () and trichome density () in mungbean were identified using recombinant inbred line (RIL) populations (PMR-1×Pusa Baisakhi) on chromosome 7. Functional analysis of QTL region identified 35 candidate genes for SPAD value (16 No) and trichome (19 No) traits.

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Article Synopsis
  • Yellow mosaic disease (YMD) poses a significant challenge to mungbean production, and research on short-duration genotypes helps mitigate heat stress during summer crops.
  • A genome-wide association study (GWAS) involving 132 mungbean genotypes identified 31,953 single nucleotide polymorphisms (SNPs) across the genome, uncovering genetic diversity and significant traits related to flowering time and disease resistance.
  • Candidate genes linked to various traits were identified, including those for YMD resistance and flowering time, providing insights for future marker-assisted breeding efforts to develop improved mungbean varieties.
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Heat shock transcription factors (HSFs) contribute significantly to thermotolerance acclimation. Here, we identified and cloned a putative HSF gene () of 1218 nucleotide (acc. no.

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Salinity or salt stress has deleterious effects on plant growth and development. It imposes osmotic, ionic, and secondary stresses, including oxidative stress on the plants and is responsible for the reduction of overall crop productivity and therefore challenges global food security. Plants respond to salinity, by triggering homoeostatic mechanisms that counter salt-triggered disturbances in the physiology and biochemistry of plants.

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Article Synopsis
  • Climate change significantly affects agricultural crop production, especially wheat, rice, and maize, raising major concerns.
  • The study analyzed wheat transcriptome data to identify heat stress-responsive long non-coding RNAs (lncRNAs), revealing 10,965 lncRNAs with 153, 143, and 211 differentially expressed transcripts during specific heat-stress timeframes.
  • Findings include 4098 lncRNAs targeted by 119 miRNAs in response to environmental stresses and insight into hub genes involved in heat stress, supporting future development of resilient wheat cultivars.
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Glutathione (GSH) is an abundant tripeptide that can enhance plant tolerance to biotic and abiotic stress. Its main role is to counter free radicals and detoxify reactive oxygen species (ROS) generated in cells under unfavorable conditions. Moreover, along with other second messengers (such as ROS, calcium, nitric oxide, cyclic nucleotides, etc.

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The seed size and shape in lentil ( Medik.) are important quality traits as these influences the milled grain yield, cooking time, and market class of the grains. Linkage analysis was done for seed size in a RIL (F) population derived by crossing L830 (20.

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Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet. Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity. Here, to explore the effect of different thermal processing methods, , hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.

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Market class, cooking time, quality, and milled grain yield are largely influenced by the seed size and shape of the lentil ( Medik.); thus, they are considered to be important quality traits. To unfold the pathways regulating seed size in lentils, a transcriptomic approach was performed using large-seeded (L4602) and small-seeded (L830) genotypes.

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This study reports the identification of a unique lentil ( Medik.) genotype L4717-NM, a natural mutant (NM) derived from a variety L4717, producing brown, black, and spotted seed-coat colored seeds in a single plant, generation after generation, in different frequencies. The genetic similarity of L4717 with that of L4717-NM expressing anomalous seed-coat color was established using 54 SSR markers.

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Microgreens have been used for raw consumption and are generally viewed as healthy food. This study aimed to optimize the yield parameters, shelf life, sensory evaluation and characterization of total aerobic bacteria (TAB), yeast and mold (Y&M), Escherichia coli, Salmonella spp., and Listeria spp.

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Dry root rot () is an important disease of lentils ( Medik.).To gain an insight into the molecular aspects of host-pathogen interactions, the RNA-seq approach was used in lentils following inoculation with .

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Pearl millet is a nutrient dense and gluten free cereal, however it's flour remains underutilized due to the onset of rancidity during its storage. To the best of our knowledge, processing methods, which could significantly reduce the rancidity of the pearl millet flour during storage, are non-existent. In this study, pearl millet grains were subjected to a preliminary hydro-treatment (HT).

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Mungbeans and lentils are relatively easily grown and cheaper sources of microgreens, but their phytonutrient diversity is not yet deeply explored. In this study, 20 diverse genotypes each of mungbean and lentil were grown as microgreens under plain-altitude (Delhi) and high-altitude (Leh) conditions, which showed significant genotypic variations for ascorbic acid, tocopherol, carotenoids, flavonoid, total phenolics, DPPH (1, 1-diphenyl-2-picrylhydrazyl), FRAP (ferric-reducing antioxidant power), peroxide activity, proteins, enzymes (peroxidase and catalase), micronutrients, and macronutrients contents. The lentil and mungbean genotypes L830 and MH810, respectively, were found superior for most of the studied parameters over other studied genotypes.

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The rising demand for popcorn necessitates improving the popping quality with higher yield of popcorn cultivars. Towards this direction several Quantitative Traits Loci (QTLs) for popping traits have been identified. However, identification of accurate and consistent QTLs across different genetic backgrounds and environments is necessary to effectively utilize the identified QTLs in marker-assisted breeding.

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Pearl millet is considered as 'nutri-cereal' because of high nutrient density of the seeds. The grain has limited use because of low keeping quality of the flour due to the activities of rancidity causing enzymes like lipase, lox, pox and PPO. Among all the enzymes, lipase is most notorious because of its robust nature and high activity under different conditions.

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