Anatomical study of perigastric fascial space and guidance for laparoscopic approach.

Objective: To study the anatomic characteristics of gastric peritoneum fascia space and provide a safe surgical approach for laparoscopic radical gastrectomy.

Method: The morphological characteristics of perigastric fascia and fascial space and the course of important blood vessels were observed and studied in 2 fresh adult cadavers, 5 formalin immersed cadavers and 56 patients undergoing total gastrectomy. The hemoglobin, albumin, prealbumin, total protein, operation time, intraoperative blood loss, number of lymph node dissection and the incidence of complications before and after laparoscopic total gastrectomy with different approaches were statistically analyzed.

Phase unwrapping error identification and suppression method in φ-OTDR systems based on PELT-VMD-ARIMA.

In phase-sensitive optical time-domain reflectometer (-OTDR) systems, phase unwrapping errors can distort vibration information. To address this issue, a phase unwrapping error identification and suppression method combining pruned exact linear time (PELT) changepoint detection, variational mode decomposition (VMD), and autoregressive integrated moving average (ARIMA) models, termed PELT-VMD-ARIMA, is proposed. Firstly, the principle of the proposed method is introduced, and its effectiveness is verified through a series of numerical simulation experiments.

Ciliogenesis requires sphingolipid-dependent membrane and axoneme interaction.

Cilium formation and regeneration requires new protein synthesis, but the underlying cytosolic translational reprogramming remains largely unknown. Using ribosome footprinting, we performed global translatome profiling during cilia regeneration in and uncovered that flagellar genes undergo an early transcriptional activation but late translational repression. This pattern guided our identification of sphingolipid metabolism enzymes, including serine palmitoyltransferase (SPT), as essential regulators for ciliogenesis.