The critical impacts of pyrochar during 2,4,6-trichlorophenol photochemical remediation process: Cooperation between persistent free radicals and oxygenated functional groups.

The widespread use of polychlorophenols poses enormous environmental challenges. Biochar has the potential to accelerate the transformation of polychlorophenols. But the biochar-triggered photochemical decomposition mechanism of polychlorophenols still remains unclear.

One-Pot Synthesis of Enzyme and Antibody/CaHPO Nanoflowers for Magnetic Chemiluminescence Immunoassay of .

In this study, through a bioinspired strategy, the horseradish peroxidase (HRP) and antibody (Ab) were co-embedded into CaHPO to prepare HRP-Ab-CaHPO (HAC) bifunctional hybrid nanoflowers by one-pot mild coprecipitation. The as-prepared HAC hybrid nanoflowers then were utilized as the signal tag in a magnetic chemiluminescence immunoassay for application in the detection of (). The proposed method exhibited excellent detection performance in the linear range of 10-10 CFU/mL, with the limit of detection (LOD) of 10 CFU/mL.

Biomimetic synthesis of protein-DNA-CaHPO hybrid nanosheets for biosensing: Detection of thrombin as an example.

Traditional strategies for coupling of proteins with DNA involve the additional modifications on protein or DNA to construct protein-DNA conjugates, resulting in complex or time-consuming coupling process. This study presented a biomimetic synthesis strategy to elaborately synthesize a new type of biomolecule-inorganic hybrid nanosheets. Horseradish peroxidase (HRP) and DNA aptamer can be easily combined with CaHPO via coprecipitation simultaneously to form all-inclusive HRP-aptamer-CaHPO hybrid (HAC) nanosheets integrating bifunction of biorecognition and signal amplification, which was proceeded in the green environment at room temperature and required no additional modifications on CaHPO, protein and DNA.

One-Pot Synthesis of HRP&SA/ZIF-8 Nanocomposite and Its Application in the Detection of Insecticidal Crystalline Protein Cry1Ab.

This study reported the functionality integration of zeolitic imidazolate framework-8 (ZIF-8) with horseradish peroxidase (HRP) and streptavidin (SA) for the synthesis of a HRP&SA/ZIF-8 nanocomposite through one-pot coprecipitation. The synthesized HRP&SA/ZIF-8 nanocomposite was then employed as the ideal signal tag for application in the enzyme-linked immunosorbent assay (ELISA) and exhibited excellent sensitivity, selectivity and accuracy in the detection of insecticidal crystalline (Cry) protein Cry1Ab as a transgenic biomarker with a detection limit of 4.8 pg/mL.

Bioinspired synthesis of protein-posnjakite organic-inorganic nanobiohybrid for biosensing applications.

This study demonstrated a facile, green and bioinspired approach to synthesize protein-posnjakite nanobiohybrid with rod-assembled hollow shuttle-like structure. Through the one-pot mild coprecipitation process, the inorganic mineral posnjakite (Cu(SO) (OH)·HO) served as a nanocarrier to efficient co-immobilization of recognition protein (streptavidin) and enzyme (horseradish peroxidase) for signal amplification, which avoids tedious linking or purification procedures and significantly simplifies the synthetic process. This nanobiohybrid was then utilized as the signal tag for immunoassays and presented excellent performance for the detection of insecticidal crystalline (Cry) protein Cry1Ab, in the linear range of 0.

Synthesis of Zn-based metal-organic frameworks in ionic liquid microemulsions at room temperature.

For the first time, Zn-metal-organic frameworks (Zn-MOFs) were prepared using Zn and the aromatic ligand 1,3,5-benzenetricarboxylic acid (BTC) in ionic liquid microemulsions stabilized by the surfactant TX-100. This proposed environmentally friendly approach to synthesize Zn-MOFs is simple, requires no energy input, and operates at room temperature. The synthesized Zn-MOFs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), single crystal X-ray diffraction and powder X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FT-IR).

Highly photoluminescent carbon dots derived from linseed and their applications in cellular imaging and sensing.

Carbon dots (CDs) synthesized from natural organic precursors, such as glucose, citric acid, glycerol, and chitosan, have attracted great interest since natural organic precursors provide abundant carbon sources, a variety of heteroatoms for doping (such as N, S, and P) and good biocompatibility. However, previous approaches utilized organic solvents during synthesis procedures, which limited their widespread development in biomedical applications. Herein, the facile synthesis of a new type of bright CDs through an eco-friendly method that employs linseed as a natural precursor has been reported.

Mitochondrial-targeted multifunctional mesoporous Au@Pt nanoparticles for dual-mode photodynamic and photothermal therapy of cancers.

In the conventional non-invasive cancer treatments, such as photodynamic therapy (PDT) and photothermal therapy (PTT), light irradiation is precisely focused on tumors to induce apoptosis via the generation of reactive oxygen species (ROS) or localized heating. However, overconsumption of oxygen and restricted diffusion distance of ROS limit the therapeutic effects on hypoxic tumors. Herein, we developed a platform for the rapid uptake of multifunctionalized Au@Pt nanoparticles (NPs) by mitochondria in cancer cells.

MnO Nanotube-Based NanoSearchlight for Imaging of Multiple MicroRNAs in Live Cells.

Sensitive assay and imaging of multiple low-abundance microRNAs (miRNAs) in living cells remain a grand challenge. Herein, based on polyelectrolyte-induced reduction, a facile approach has been proposed to synthesize novel MnO nanotubes. Owing to the remarkably strong fluorescence quenching ability, low cytotoxicity, and excellent colloid stability, the as-prepared MnO nanotubes showed great potential for simultaneous detection and imaging of multiple miRNAs in vitro and in situ in living cells for the first time.

"On-Off-On" fluorescence sensor based on g-CN nanosheets for selective and sequential detection of Ag and S.

Detection of silver (Ag) and sulfide (S) ions is important because their presence in large amounts can cause many diseases. In this study, a novel, simple, "on-off-on" fluorescence sensor based on g-CN nanosheets for sequential detection of Ag and S was designed. The fluorescence signal of the g-CN nanosheets is quenched because Ag chelates with the N of the g-CN nanosheets, leading to photoinduced electron transfer from the sheets to Ag.

One-pot bioinspired synthesis of all-inclusive protein-protein nanoflowers for point-of-care bioassay: detection of E. coli O157:H7 from milk.

Protein-protein conjugates play a vital role in bioassays with their inherent functions of biological recognition and signal amplification. Herein, a one-pot green method for synthesis of all-inclusive protein-protein nanoflowers has been developed. The protein-protein nanoflowers integrate both essential functions of biological recognition and signal amplification, and they were used as ideal signal labels for the sensitive point-of-care detection of Escherichia coli O157:H7.

Bioinspired Synthesis of All-in-One Organic-Inorganic Hybrid Nanoflowers Combined with a Handheld pH Meter for On-Site Detection of Food Pathogen.

With a mild elaborately bioinspired one-pot process, Con A-GOx-CaHPO4 nanoflowers are prepared. Employing the as-prepared all-in-one hybrid nanoflowers as signal tags, a simple but potentially powerful amplification biosensing technology for the detection of food pathogen with excellent simplicity, portability, sensitivity, and adaptability is achieved.