The efficacy of Acanthamoeba cyst kill and effects upon contact lenses of a novel ultraviolet lens disinfection system.

Purpose: To assess the efficacy of a novel ultraviolet (UV) lens device on the killing of Acanthamoeba cysts and the impact of efficacious doses of UV upon soft contact lens parameter and material characteristics.

Design: Prospective, in vitro, experimental study of a device.

Methods: A UV lens device was constructed and used to expose Acanthamoeba cysts to various levels of UV irradiation.

A high-throughput microtiter plate based method for the determination of peracetic acid and hydrogen peroxide.

Peracetic acid is gaining usage in numerous industries who have found a myriad of uses for its antimicrobial activity. However, rapid high throughput quantitation methods for peracetic acid and hydrogen peroxide are lacking. Herein, we describe the development of a high-throughput microtiter plate based assay based upon the well known and trusted titration chemical reactions.

The use of chromophore and fluorophore degradation to quantitate UV dose: FD&C dyes as chemical identicators for UV sterilization.

The accurate measurement of ultraviolet (UV) irradiation, especially within a container or vessel is one of the challenges facing the broad implementation of UV sterilization. Currently, biological indicators are the best method to determine whether an applied UV dose has the necessary efficacy to achieve sterilization. To overcome some of the challenges of using a biological indicator, chemical indicators based upon the degradation of food, drug and cosmetic (FD&C) dyes were developed.