Publications by authors named "Ran-Ran Su"
Glenea cantor (Fabricius) is an important forest pest that mainly attacks kapok trees, breaking down cellulose and lignin through 3 enzyme activities: endoglucanase, filter paper enzyme, and cellobiase. In this study, we unveiled the cloning and expression of 10 endoglucanase genes, GcEGase5A1, GcEGase5A2, GcEGaseZ2, GcEGaseZ3, GcEGaseZ4, GcEGaseZ5, GcEGaseZ7, GcEGaseZ8, GcEGaseZ9, and Cellulase, all of which exhibit enzymatic activities in G. cantor.
View Article and Find Full Text PDFBackground: The production of succinic acid (SA) from biomass has attracted worldwide interest. Saccharomyces cerevisiae is preferred for SA production due to its strong tolerance to low pH conditions, ease of genetic manipulation, and extensive application in industrial processes. However, when compared with bacterial producers, the SA titers and productivities achieved by engineered S.
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- The study investigates the intestinal bacteria of longhorn beetles, focusing on their diversity and community structure, specifically looking for bacteria that can degrade cellulose from their host, kapok trees.
- A total of 563,201 valid sequences were analyzed, revealing high bacterial diversity, with Proteobacteria as the dominant phyla and several genera identified, including nine with cellulose-degrading capabilities.
- Five strains of cellulose-degrading bacteria were isolated, with one strain showing high cellulase activity, suggesting these bacteria could be targeted for pest control and resource exploitation in agricultural settings.
Article Synopsis
- - The study focuses on how neuropeptides and their receptors regulate physiological processes in the insect Phauda flammans, a plant-eating pest found in Asia.
- - Researchers sequenced the genomes of male and female insects, identifying 99,386 unigenes, with 156 genes up-regulated and 61 down-regulated, as well as 15 neuropeptides and 66 neuropeptide receptor genes.
- - This research is the first of its kind for P. flammans, offering new insights into its molecular biology, which can help understand its biology and management.
Article Synopsis
- Researchers aimed to improve the multiple stress-tolerance of Saccharomyces cerevisiae, critical for efficient ethanol production, through genetic engineering, which is less time-consuming than traditional methods.
- Twenty-eight differentially expressed genes were identified in a stress-tolerant strain, with six selected for further research, including genes with known and unknown functions, as well as transcription factors.
- Using CRISPR/Cas9 technology, the selected genes were edited, successfully enhancing stress tolerance and ethanol production, with the engineered strain E-158-ENA5 showing a significant increase in ethanol yield compared to its predecessor.
Kapok is the main host of (Fabricius), which causes serious damage and is difficult to control. In severe cases, it often causes the kapok trees to die continuously, which seriously affects the results of urban landscaping. To provide reference for the functional research on related genes in , we screened the stable expression of candidate reference genes at different developmental stages (i.
View Article and Find Full Text PDFWe validated and used a high-performance liquid chromatography procedure for the determination of four different amino acid neurotransmitters in cultured rat neurons and used culture medium. Samples were derivatized using 2,4-dinitrofluorobenzene and the amino acids were separated on a C18 column. The method yielded good reproducibility and sensitivity for the quantification of the four free amino acid neurotransmitters, with average recovery factors of 80.
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