Publications by authors named "Ran Shachar"
Background: N6-methyladenosine (m6A) is the most abundant mRNA modification, and controls mRNA stability. m6A distribution varies considerably between and within species. Yet, it is unclear to what extent this variability is driven by changes in genetic sequences ('cis') or cellular environments ('trans') and via which mechanisms.
View Article and Find Full Text PDFArticle Synopsis
- N-acetylcytidine (acC) is a conserved RNA modification primarily found in tRNA and rRNA, and research is expanding to its role in eukaryotic mRNA.
- The study introduces acC-seq, a method to map acC at a single-nucleotide level, revealing that while acC is absent in human and yeast mRNAs, it can be induced through the overexpression of specific acetyltransferase complexes.
- In archaea, significant levels of acC were found in various RNA types, especially with increased temperatures, which also affected growth in specific strains, suggesting a potential temperature-adaptive role for acC.
Article Synopsis
- N6-methyladenosine (mA) is a key modification in mRNA affecting development and disease, but quantifying it has been challenging due to limited methodologies.
- A new technique called MAZTER-seq allows for precise profiling of m6A at the single-nucleotide level, enabling the validation of existing approaches and tracking of mA changes across various biological contexts like yeast gametogenesis and mammalian differentiation.
- The study reveals that m6A levels follow a predictable coding system, explaining a significant portion of the variability in methylation and facilitating predictions about how mA is gained or lost throughout evolution.
Background: Medication nonadherence is a major problem in health care, imposing poor clinical outcomes and a heavy financial burden on all stakeholders. Current methods of medication adherence assessment are severely limited: they are applied only periodically, do not relate to actual pill intake, and suffer from patient bias due to errors, misunderstanding, or intentional nonadherence. ReX is an innovative medication management system designed to address poor patient adherence and enhance patient engagement with their therapy.
View Article and Find Full Text PDF