Bioengineering vascularization.

This Review explores the rapidly evolving field of bioengineered vasculature, a key area of focus in tissue engineering and regenerative medicine. The broad relevance of this topic is attributed to its impacts on a wide range of biological processes, enabling studies in tissue development, fundamental biology and drug discovery, and the applications in tissue engineering and regenerative medicine. We outline the design criteria for bioengineered vasculature and the methodologies for constructing these systems by self-assembly and in microfluidics, organs-on-a-chip and macroscale tubular systems that often rely on biofabrication approaches such as 3D printing.

Cell driven elastomeric particle packing in composite bioinks for engineering and implantation of stable 3D printed structures.

Geometric and structural integrity often deteriorate in 3D printed cell-laden constructs over time due to cellular compaction and hydrogel shrinkage. This study introduces a new approach that synergizes the advantages of cell compatibility of biological hydrogels and mechanical stability of elastomeric polymers for structure fidelity maintenance upon stereolithography and extrusion 3D printing. Enabling this advance is the composite bioink, formulated by integrating elastomeric microparticles from poly(octamethylene maleate (anhydride) citrate) (POMaC) into biologically derived hydrogels (fibrin, gelatin methacryloyl (GelMA), and alginate).

Visible Light in the Treatment of Acne Vulgaris.

Acne vulgaris, a prevalent dermatological disorder, frequently affects individuals' self-perception and general psychosocial functioning. The growing demand for home-based light therapy devices prompted a systematic review to assess the efficacy of visible light in acne treatment. Overall, 35 studies were included, representing 1185 cases of acne vulgaris (mean age: 23.

Automated fabrication of a scalable heart-on-a-chip device by 3D printing of thermoplastic elastomer nanocomposite and hot embossing.

The successful translation of organ-on-a-chip devices requires the development of an automated workflow for device fabrication, which is challenged by the need for precise deposition of multiple classes of materials in micro-meter scaled configurations. Many current heart-on-a-chip devices are produced manually, requiring the expertise and dexterity of skilled operators. Here, we devised an automated and scalable fabrication method to engineer a Biowire II multiwell platform to generate human iPSC-derived cardiac tissues.

Ceftazidime resistance in Pseudomonas aeruginosa is multigenic and complex.

Pseudomonas aeruginosa causes a wide range of severe infections. Ceftazidime, a cephalosporin, is a key antibiotic for treating infections but a significant proportion of isolates are ceftazidime-resistant. The aim of this research was to identify mutations that contribute to resistance, and to quantify the impacts of individual mutations and mutation combinations.

Epidural Fat and Perineural Adipose Tissue Septic Emboli Mimicking Peripheral Nerve Sheath Tumor in a Dog.

. A 9-year-old 35.6 kg (90 lb) female neutered German Shepherd dog was admitted due to progression of tetraparesis.

Rapid fluoroquinolone resistance detection in using mismatch amplification mutation assay-based real-time PCR.

Antimicrobial resistance (AMR) is an ever-increasing global health concern. One crucial facet in tackling the AMR epidemic is earlier and more accurate AMR diagnosis, particularly in the dangerous and highly multi-drug-resistant ESKAPE pathogen, . We aimed to develop two SYBR Green-based mismatch amplification mutation assays (SYBR-MAMAs) targeting GyrA T83I (248) and GyrA D87N, D87Y and D87H (259).

Express Yourself: Quantitative Real-Time PCR Assays for Rapid Chromosomal Antimicrobial Resistance Detection in Pseudomonas aeruginosa.

The rise of antimicrobial-resistant (AMR) bacteria is a global health emergency. One critical facet of tackling this epidemic is more rapid AMR diagnosis in serious multidrug-resistant pathogens like Pseudomonas aeruginosa. Here, we designed and then validated two multiplex quantitative real-time PCR (qPCR) assays to simultaneously detect differential expression of the resistance-nodulation-division efflux pumps MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY-OprM, the AmpC β-lactamase, and the porin OprD, which are commonly associated with chromosomally encoded AMR.

Treatment of Provoked Vulvodynia: A Systematic Review.

Background: Treatment recommendations for provoked vulvodynia (PVD) are based on clinical experiences and there is a need for systematically summarizing the controlled trials in this field.

Aim: To provide an overview of randomized controlled trials and non-randomized studies of intervention for PVD, and to assess the certainty of the scientific evidence, in order to advance treatment guidelines.

Data Sources: The search was conducted in CINAHL (EBSCO), Cochrane Library, Embase (Embase.

The Effects of Sub-inhibitory Antibiotic Concentrations on : Reduced Susceptibility Due to Mutations.

chronically infects in the lungs of people with cystic fibrosis and other forms of lung disease. Infections are treated with antibiotics, but over time, the bacteria acquire mutations that reduce their antibiotic susceptibility. The effects of inhibitory amounts of antibiotics in selecting for antibiotic-resistant mutants have been well studied.

Programmed assembly of bespoke prototissues on a microfluidic platform.

The precise assembly of protocell building blocks into prototissues that are stable in water, capable of sensing the external environment and which display collective behaviours remains a considerable challenge in prototissue engineering. We have designed a microfluidic platform that enables us to build bespoke prototissues from predetermined compositions of two types of protein-polymer protocells. We can accurately control their size, composition and create unique Janus configurations in a way that is not possible with traditional methods.

Algae Adhesion onto Silicone is Sensitive to Environment-Induced Surface Restructuring.

Resistance to algae contamination is an important characteristic of insulators used in overhead power distribution in coastal environments. It is therefore important to understand the parameters governing algae adhesion onto polymer insulator materials such as silicone. Flow cell-based shear experiments were conducted in order to characterize the adhesion strength of algae onto polydimethylsiloxane surfaces, comparing fresh polymer substrates with those that have been soaked in water and saline solutions for 1 month.

A plug-and-play modular microcapillary platform for the generation of multicompartmental double emulsions using glass or fluorocarbon capillaries.

Although multiple emulsions have a wide range of applications in biology, medicine, chemistry and cosmetics, the use of microfluidic devices to generate them remains limited to specialist laboratories. This is because of the expertise required to design and operate these technologies. Here we show a plug-and-play microcapillary platform for the generation of multicompartmental double emulsions which only requires a low cost 3D printer for fabrication and syringe pumps for operation.

Emergence and impact of oprD mutations in Pseudomonas aeruginosa strains in cystic fibrosis.

Background: Antimicrobial resistance in cystic fibrosis (CF) Pseudomonas aeruginosa airway infection is complex and often attributed to chromosomal mutations. How these mutations emerge in specific strains or whether particular gene mutations are clinically informative is unclear. This study focused on oprD, which encodes an outer membrane porin associated with carbapenem resistance when it is downregulated or inactivated.

Designing success: Applying Cognitive Work Analysis to optimise a para sport system.

Within the last decade, Para sport has experienced increased growth, global popularity, and scientific research. Likewise, there is increasing application of systems ergonomics methods to optimise sports performance. Despite this, few studies have attempted to analyse Para sport as a complex system.

Scientific and Ethical Issues in Exporting Welfare Findings to Different Animal Subpopulations: The Case of Semi-Captive Elephants Involved in Animal-Visitor Interactions (AVI) in South Africa.

Elephants are charismatic, cognitively highly-developed animals, whose management conditions can vary along a "wild-captive continuum." Several protocols have been proposed for the assessment of zoo elephants' welfare. It is important to investigate the possible limitations, if any, of extending findings from zoo elephants to conspecifics in a different dynamic in said "wild-captive continuum.

Genomic and phenotypic comparison of environmental and patient-derived isolates of suggest that antimicrobial resistance is rare within the environment.

Patient-derived isolates of the opportunistic pathogen are frequently resistant to antibiotics due to the presence of sequence variants in resistance-associated genes. However, the frequency of antibiotic resistance and of resistance-associated sequence variants in environmental isolates of has not been well studied. Antimicrobial susceptibility testing (ciprofloxacin, ceftazidime, meropenem, tobramycin) of environmental (=50) and cystic fibrosis (=42) isolates was carried out.

Prevalence and correlates of autism spectrum disorder in Qatar: a national study.

Background: Few epidemiological data on autism spectrum disorders (ASD) exist for Arabic countries. We conducted the first survey of ASD in Qatar, a population with high consanguinity level.

Methods: This cross-sectional survey was conducted from 2015 to 2018 in Qatar school-age children (N = 176,960) from national and immigrant families.

Development and standardization of the University of Cincinnati Auditory Processing Inventory (UCAPI).

Objective: A literature review found that questionnaires for persons with problems of listening seeking assessment of auditory processing skills are not applicable for adolescents and adults. We developed a questionnaire designed for adolescents and adults seeking evaluation of problems with listening or following diagnosis to determine the subject's status.

Design: Following a model described by Del Zoppo, Sanchez, and Lind, a 34-item questionnaire was developed that investigates auditory processing abilities in six common areas of complaint in Auditory processing disorders (APD) (Keith, et al.

Transmission of bacteria in bronchiectasis and chronic obstructive pulmonary disease: Low burden of cough aerosols.

Background And Objective: Aerosol transmission of Pseudomonas aeruginosa has been suggested as a possible mode of respiratory infection spread in patients with cystic fibrosis (CF); however, whether this occurs in other suppurative lung diseases is unknown. Therefore, we aimed to determine if (i) patients with bronchiectasis (unrelated to CF) or chronic obstructive pulmonary disease (COPD) can aerosolize P. aeruginosa during coughing and (ii) if genetically indistinguishable (shared) P.

Correction: Within-host whole genome analysis of an antibiotic resistant Pseudomonas aeruginosa strain sub-type in cystic fibrosis.

[This corrects the article DOI: 10.1371/journal.pone.

Haemopedia RNA-seq: a database of gene expression during haematopoiesis in mice and humans.

During haematopoiesis, haematopoietic stem cells differentiate into restricted potential progenitors before maturing into the many lineages required for oxygen transport, wound healing and immune response. We have updated Haemopedia, a database of gene-expression profiles from a broad spectrum of haematopoietic cells, to include RNA-seq gene-expression data from both mice and humans. The Haemopedia RNA-seq data set covers a wide range of lineages and progenitors, with 57 mouse blood cell types (flow sorted populations from healthy mice) and 12 human blood cell types.