Antimicrobial potential of secondary metabolites and DNA gyrase B blocking molecules produced by a halophilic bacterium Virgibacillus salarius (MML1918).

Aim: The present study aims to determine the antimicrobial potential of Virgibacillus salairus (MML1918) against human pathogens and its in-vitro and in-silico properties.

Methods And Results: In this present study, totally 63 halophilic bacterial cultures were obtained and cultivated in nutrient broth medium containing 8% NaCl and the metabolites, were extracted using ethyl acetate and screened for their antimicrobial property by cell viability assay against 12 pathogenic bacteria and fungi, among 63 halophilic bacteria the Vir. salaries (MML1918) found to be the best producer for secondary metabolites production against clinical pathogens.

Optical Fiber-Based Steady State and Fluorescence Lifetime Spectroscopy for Rapid Identification and Classification of Bacterial Pathogens Directly from Colonies on Agar Plates.

Fluorescence spectroscopy was examined as a potential technique for identification and classification of bacterial pathogens. Colonies of Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae on agar plates were measured directly using a laboratory spectrofluorimeter coupled with optical fiber. Steady state fluorescence spectra were collected following excitation at 280 nm (tryptophan) and 380 nm (NADH).

Synthesis of 7-oxabicyclo[2.2.1]hept-5-en-2-yl derivatives and their screening for antimicrobial and antioxidant properties.

Novel 7-oxabicyclo[2.2.1]hept-5-en-2-yl derivatives have been synthesized using boron trifluoride diethyl etherate catalyzed Diels-Alder reaction.

Synthesis of quinoline derivatives for fluorescent imaging certain bacteria.

Highly fluorescent quinoline derivatives were synthesized using Sc(OTf)(3) catalyzed imino Diels-Alder reaction. Both the aromatic and their analogous tetradehydroquinoline derivatives were explored for the detection of bacteria using fluorescent imaging studies. Surprisingly the aromatic quinoline derivatives show a remarkable fluorescent response that can be useful in the detection of both gram positive and gram negative bacteria even at a concentration in the range of 0.