The G-Force Conundrum in Platelet-Rich Fibrin Generation: Management of a Problem Hidden in Plain Sight.

Aim: A force of 400 g at 2700 revolutions per minute (RPM) results in an optimum leukocyte and platelet-rich fibrin (L-PRF). Most of centrifuges with varying characteristics generate a g-force in excess of 700 g at 2700 RPM. In this context, the study explores the effect of the original centrifugation protocol and a modified protocol tailor-made to lower the RPM to generate a g-force of ~ 400 g on platelet concentration, clot size and growth factors release in L-PRF prepared in two different commercially available centrifuges.

Comparative Evaluation of Efficacy between Recombinant Human Bone Morphogenetic Protein-2 Impregnated with Absorbable Sponge and Platelet-Rich Fibrin in the Treatment of Grade II Furcation Defects: A Randomized Controlled Trial.

Aim And Objectives: The objective of the study was to clinically and radiographically compare and evaluate the regenerative potential of recombinant human bone morphogenetic protein-2 (RhBMP-2) impregnated with absorbable collagen sponge and platelet-rich fibrin (PRF) in the treatment of Grade II furcation defects.

Patients And Methods: Thirty-two subjects were randomly assigned to each of the following groups: bone morphogenetic protein (BMP) group and PRF group, with one defect/subject. Sixteen Grade II furcation defects were treated with RhBMP-2 impregnated with absorbable collagen sponge in the BMP group and the remaining 16 defects were treated with PRF in the PRF group.

Teeth loss and its association with locomotive syndrome among patients visiting the outpatient department of a dental school in Mahbubnagar, India-A cross sectional study.

Background: Locomotive syndrome (LoS) is condition wherein mobility functions such as sit-to-stand or gait are declined due to locomotive organ impairment. The important risk factors for the LoS are ageing and physical factors. Physical factors significantly associated with dental status Aim: To investigate the relationships between teeth lost and locomotive syndrome.

Efficacy of recombinant human fibroblast growth factor 2 impregnated absorbable collagen membrane in the treatment of Miller's Class I and II gingival recession defects Preliminary results from the first in human clinical trial.

Aims: This study was a single-arm trial to obtain preliminary data on the efficacy of collagen membranes impregnated with recombinant human fibroblast growth factor-2 (rhFGF-2) in the treatment of Miller's Class I and II gingival recessions.

Materials And Methods: Twenty-one individuals (34 sites) presenting with localized Miller's Class I and II gingival recessions were included in this study. Following a standard surgical protocol, rhFGF-2-impregnated membranes were placed in sites with gingival recession.

Lateral osteoperiosteal flap versus lateral pedicle flap in the treatment of class III gingival recession: A single-center, open-label trial.

Aims: The aim of this study was to compare outcomes of lateral osteoperiosteal flap (OPF) and lateral pedicle flap (LPF) in the treatment of Miller's Class III gingival recession.

Materials And Methods: Twenty-two anterior maxillary and mandibular sites from 16 participants requiring mucogingival surgery for Miller's Class III gingival recession were included in the study. Eleven sites each were assigned to two groups.

Free gingival grafts vs mucosal excision in increasing the amount of keratinized mucosa during exposure of submerged orthodontic implants: a comparative, split-mouth study.

Objectives: The aim of the present study was to compare the clinical outcomes after using free gingival grafts (FGGs) and mucosal excision during exposure of submerged orthodontic implants.

Method And Materials: Bilateral sites in 28 subjects were divided into two groups: In the FGG group, FGG augmentation was performed around the orthodontic implants, and in the mucosal excision group, a periosteal bed was made after mucosal excision to promote attached mucosa with no additional soft-tissue augmentation. Outcomes measured during the course of the study were the width of attached mucosa, soft tissue regrowth, degree of inflammation, oral debris, and shrinkage of the attached mucosa around the orthodontic implants over a period of 3 months.

Regenerative Capacity of Leukocyte-rich and Platelet-rich Fibrin in Indirect Sinus Elevation Procedure May be Dependent on Model-Specific Modification of the Centrifugation Cycle.

Context: To compare optical density (OD) and fibrinogen content of leukocyte-rich and platelet-rich fibrin (L-PRF) generated by standard protocol (2700 rotations per minute [RPM] for 12 min) versus relative centrifugal force (RCF)-adjusted protocol across two widely used laboratory centrifuges with swing-out rotors.

Aims: Centrifuges for PRF production generate forces in excess of 800 g. The study aimed to evaluate OD, fibrinogen content and effectiveness in bone-added osteotome sinus floor elevation (BAOSFE) of leukocyte-rich and platelet-rich fibrin (L-PRF) generated by the standard protocol (2700 RPM for 12 min) versus a RCF-adjusted protocol to generate precisely 400 g of force across two centrifuges with swing-out rotors.

Palatal pyogenic granuloma: An unusual complication following mucogingival surgery for alveolar socket preservation.

We report a case of palatal pyogenic granuloma following mucogingival surgery for alveolar socket preservation. A 24-year-old systemically healthy female underwent a pediculated palatal pedicle graft procedure to achieve soft tissue augmentation over a grafted maxillary anterior extraction site. After 1 month, a 15 mm × 20 mm exophytic growth extending from the palatal donor site to distance of 3-4 mm from the extraction socket was observed.

Autogenous Bone Ring Transplant vs Autologous Growth Factor-Enriched Bone Graft Matrix In Extraction Sockets With Deficient Buccal Bone: A Comparative Clinical Study.

Purpose: The purpose of this study was to compare the efficacies of autogenous bone ring and autologous growth factor-enriched bone graft matrix as graft materials in extraction sockets.

Materials And Methods: Subjects (n = 34) requiring extraction of a single incisor or premolar in a type II socket were segregated into two groups: BR and AFG. Autogenous bone ring transplant and autologous fibrin glue with particulate bone graft (sticky bone) was used in subjects under the BR and AFG groups, respectively, for socket augmentation.

Non-Cross-Linked Versus Cross-Linked Collagen Membrane In Maxillary Sinus Perforation Repair: A Comparative Histologic Study In A Rat Model.

Purpose: To compare and evaluate maxillary sinus perforation repair, bone regeneration, and membrane degradation with cross-linked and non-cross-linked collagen membranes in rat sinuses at 2, 4, and 10 weeks, respectively.

Materials And Methods: Fifty-one Sprague-Dawley rat models were included in the study. Bilateral maxillary sinus perforations were made with a straight bur.

Periodontally accelerated osteogenic orthodontics combined with recombinant human bone morphogenetic protein-2: An outcome assessment.

Context: Periodontally accelerated osteogenic orthodontics (PAOO) combines alveolar corticotomy, bone graft materials, and the application of orthodontic forces for rapid correction of malocclusions.

Aims: The present study aims to primarily assess differences in orthodontic treatment duration, bone quality around corticotomy sites, postoperative healing, and subjective pain when corticotomy was done conventionally and with the placement of recombinant human bone morphogenetic protein-2 (rhBMP-2).

Settings And Design: Thirty individuals participated in this study.

Trephine Core Procedure Versus Bone-Added Osteotome Sinus Floor Elevation in the Augmentation of the Sinus Floor: A Comparative Clinical and Radiographic Study.

Purpose: The aim of this study was to clinically and radiographically compare the trephine core procedure and the bone-added osteotome sinus floor elevation technique in the augmentation of the sinus floor.

Materials And Methods: A single site in each subject requiring sinus augmentation where the residual bone height was ~4 mm was randomly allocated to either the trephine core procedure or the bone-added osteotome sinus floor elevation technique. Measures of bone fill and bone height were also acquired through scans from cone beam computed tomography obtained before the sinus augmentation procedure (baseline) and before implant placement at approximately 6 months postoperatively.

Comparing the outcomes of incisions made by colorado microdissection needle, electrosurgery tip, and surgical blade during periodontal surgery: A randomized controlled trial.

Context: Electrosurgery offers many unique advantages such as hemostasis and precise tissue cutting; however, there are a number of disadvantages including thermal injury and delayed wound healing.

Aims: The aim of the present study was to compare the outcomes of incisions made by Colorado microdissection needle, electrosurgery tip, and surgical blade during periodontal surgery.

Settings And Design: Twenty-two individuals participated in this study.

Estimation of tissue and crevicular fluid oxidative stress marker in premenopausal, perimenopausal and postmenopausal women with chronic periodontitis.

Objectives: The aim of this study was to estimate tissue and gingival crevicular fluid (GCF) levels of the oxidative stress marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) in premenopausal, perimenopausal and postmenopausal women with chronic periodontitis.

Background: Oxidative stress has been implicated in the etiopathogenesis of periodontitis and menopause induces oxidative stress.

Materials And Methods: According to Stages of Reproductive Aging Workshop (STRAW) criteria, women diagnosed with periodontitis were subdivided into three groups of 31 participants each 1.

Efficacy of a Novel Zn-Substituted Monetite-Based Scaffold in the Treatment of Periodontal Osseous Defects.

Purpose: The objective of this study was to evaluate the efficacy of a zinc-substituted nanostructured monetite-based scaffold (Sil-Oss®) in the treatment of periodontal intra-bony osseous defects.

Methods: Thirty subjects participated in this study. Two sites in each subject were randomly assigned into each of the following experimental groups: Test group - open flap debridement (OFD) with Sil-Oss®; and control group - OFD with hydroxyapatite (HA) bone graft.

Effect of a Locally Delivered Probiotic-Prebiotic Mixture as an Adjunct to Scaling and Root Planing in the Management of Chronic Periodontitis.

Objectives: This study aimed to determine the efficacy of a probiotic, Saccharomyces boulardii (S. boulardii), mixed with a prebiotic, fructooligosaccharide (FOS), in the treatment of periodontal disease when used as an adjunct to non-surgical periodontal therapy (NSPT).

Methods: The study was carried out on 30 subjects who were diagnosed as having chronic periodontitis and had at least two sites in two different quadrants with ≥ 5 mm probing pocket depth (PPD).

Possible Association Between the Mallampati Score of the Oropharynx and Measures of Tongue Coating, Oral Hygiene and Periodontal Status.

Purpose: To assess oral hygiene and the gingival and periodontal disease status in subjects scored under the modified Mallampati classification (MMC) of the oropharynx.

Patients And Methods: The clinical parameters included recording MMC scores, simplified oral hygiene index (OHI-S), modified gingival index (MGI), tongue coating index (TCI) and periodontal status of the subjects. Eight additional parameters, which included percentage of sites with bleeding on probing (BOP), sites with probing depth (PD) ≥ 5 mm, tooth loss, attachment loss (AL):age ratio, diabetic status, smoking, the interplay of dental status and systemic factors (DS-SFI), and background characteristics (socioeconomic status and stress) were also assessed.

Identical mitochondrial somatic mutations unique to chronic periodontitis and coronary artery disease.

Context: The inflammatory processes involved in chronic periodontitis and coronary artery diseases (CADs) are similar and produce reactive oxygen species that may result in similar somatic mutations in mitochondrial deoxyribonucleic acid (mtDNA).

Aims: The aims of the present study were to identify somatic mtDNA mutations in periodontal and cardiac tissues from subjects undergoing coronary artery bypass surgery and determine what fraction was identical and unique to these tissues.

Settings And Design: The study population consisted of 30 chronic periodontitis subjects who underwent coronary artery surgery after an angiogram had indicated CAD.

Concomitant Correction of a Soft-Tissue Fenestration with Keratinised Tissue Augmentation By Using A Rotated Double-Pedicle Flap During Second-Stage Implant Surgery- A Case Report.

Soft tissue deficiencies and defects around dental implants have been observed frequently. Soft-tissue defects after implant procedures originate from the process of modelling of periimplant mucosa and often cause aesthetic disharmony, food debris accumulation and soft tissue shrinkage. Periimplant mucogingival surgery focuses on creating an optimum band of keratinized tissue resulting in soft tissue architecture similar to the gingiva around natural teeth.

Evaluation of the Antibacterial Effect of Silver Nanoparticles on Guided Tissue Regeneration Membrane Colonization--An in Vitro Study.

Objective: The aim of this in vitro study was to investigate the colonization and penetration of specific bacteria on nanosilver-impregnated GTR (guided tissue regeneration) membranes.

Methods: Three sets of GTR membranes were used in this study: 1) GTR-C: Plain GTR membrane as a negative control; 2) GTR-NS: GTR membrane impregnated with silver nanoparticles as the test group; 3) GTR-DOX: GTR membrane impregnated with 25% (w/w) doxycycline hydrochloride acting as a positive control. Stress-strain characteristics were calculated to determine the physical properties of the control and impregnated membranes.

Evaluation of clinical, antiinflammatory and antiinfective properties of amniotic membrane used for guided tissue regeneration: A randomized controlled trial.

Background: The objective of this study was to evaluate the antiinflammatory, antiinfective and clinical properties of amniotic membrane (AM) when used for guided tissue regeneration (GTR) in contained interdental defects.

Materials And Methods: A total of 30 subjects participated in this study. Two sites in each subject were randomly assigned into each of the following experimental groups; test group: AM with bone graft and control group: Bone graft only.

Effect of a single session of antimicrobial photodynamic therapy using indocyanine green in the treatment of chronic periodontitis: a randomized controlled pilot trial.

Objective: The aim of this randomized controlled pilot study was to evaluate the effects of indocyanine green (ICG) as an adjunct to nonsurgical periodontal therapy in terms of reduction in percentage of viable bacteria and host tissue injury.

Method And Materials: The study included a small cohort of 30 subjects diagnosed with chronic periodontitis. Three sites from three different quadrants were selected and were randomly treated by (1) scaling and root planing (SRP), (2) SRP with application of 810 nm diode laser, or (3) SRP with application of 810 nm diode laser and ICG at a concentration of 5 mg/ mL.

Locally delivered antioxidant gel as an adjunct to nonsurgical therapy improves measures of oxidative stress and periodontal disease.

Purpose: The present study has two aims; firstly, it attempts to verify the presence of oxidative stress by estimating the reactive oxygen species (ROS) levels in periodontal pockets ≥5 mm as compared to controls. The second aim is to evaluate the effect of lycopene as a locally delivered antioxidant gel on periodontal health and on the gingival crevicular fluid (GCF) levels of 8-hydroxydeoxyguanosine (8-OHdG), a marker of oxidative injury.

Methods: Thirty-one subjects participated in this study.