Publications by authors named "Ramon Souza-Perera"
Ectopic expression of the Coffea canephora SERK1 homolog-induced differential transcription of genes involved in auxin metabolism and in the developmental control of embryogenesis.
Daniel Pérez-Pascual Doribet Jiménez-Guillen Hernán Villanueva-Alonzo Ramón Souza-Perera Gregorio Godoy-Hernández
Physiol Plant
August 2018
Somatic embryogenesis receptor-like kinase 1 (SERK1) is a membrane receptor that might serve as common co-regulator of plant cell differentiation processes by forming heterodimers with specific receptor-like kinases. The Coffea canephora SERK1 homolog (CcSERK1) was cloned in this work, and its early function in the transcription of embryogenesis master genes and of genes encoding proteins involved in auxin metabolism was investigated by externally manipulating its expression in embryogenic leaf explants, before the appearance of embryogenic structures. Overexpression of CcSERK1 early during embryogenesis caused an increase in the number of somatic embryos when the 55-day process was completed.
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- The mRNA differential display technique was utilized to identify genes in Habanero pepper seedlings affected by Phytophthora capsici infection, showing that multiple transcripts accumulate rather than a single gene being newly synthesized.
- Levels of the nitrate reductase (CcNR) gene increased significantly a few hours post-inoculation and were also elevated after treatment with salicylic acid and ethephon, indicating a potential protective role in response to infection.
- The study suggests that the regulation of CcNR and its enzymatic activity is linked to host defense mechanisms, influenced by both the oomycete and certain phytohormones.