A mutation in the 5,10-methylenetetrahydrofolate reductase gene is not associated with preeclampsia in women of southeast Mexico.

Background: We investigated the possible association between C677T-5,10-methylenetetrahydrofolate (MTHFR) polymorphism and preeclampsia in women from the Yucatan Peninsula in Mexico.

Methods: We performed a comparative study among three groups of subjects: 148 preeclamptic women; 177 women with normal pregnancies, and 313 healthy volunteers as control group. All subjects were genotyped for C677T polymorphism in MTHFR gene by PCR amplification and digestion of the product with Hinf I restriction enzyme.