Publications by authors named "Rami Aalto"
Tracer antibodies, which are labelled with fluorescent or other type of reporter molecules, are widely employed in diagnostic immunoassays. Time-resolved fluorescence immunoassay (TRFIA), recognized as one of the most sensitive immunoassay techniques, utilizes tracers labelled with lanthanide ion (Ln) chelates. The conventional approach for conjugating isothiocyanate (ITC) Ln-chelates to antibodies involves random chemical targeting of the primary amino group of Lys residues, requiring typically overnight exposure to an elevated pH of 9-9.
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- A novel, highly sensitive immunoassay for long cardiac troponin T (cTnT) was developed using upconverting nanoparticles to improve detection for acute myocardial infarction (AMI).
- The new assay achieved significantly lower detection (0.40 ng/L) and quantitation (1.79 ng/L) limits compared to previous methods and was effective in distinguishing between patients with non-ST elevation myocardial infarction and end-stage renal disease.
- The findings suggest that this novel assay could enhance the use of long cTnT as a specific biomarker for AMI, with promising clinical implications.