The influence of soil environmental factors such as aeration on the ecology of microorganisms involved in the mineralization and degradation of the popular soil-applied pre-emergent herbicide, metolachlor is unknown. To address this knowledge gap, we utilized DNA-based stable isotope probing (SIP) where soil microcosms were incubated aerobically or anaerobically and received herbicide treatments with unlabeled metolachlor or C-metolachlor. Mineralization of metolachlor was confirmed as noted from the evolution of CO from C-metolachlor-treated microcosms and clearly demonstrated the efficient utilization of the herbicide as a carbon source.
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