Collaborative Deep Learning for Privacy Preserving Diabetic Retinopathy Detection.

Convolutional Neural Networks (CNNs) are an emerging research area for detection of Diabetic Retinopathy (DR) development in fundus images with highly reliable results. However, its accuracy depends on the availability of big datasets to train such a deep network. Due to the privacy concerns, the strict rules on medical data limit accessibility of images in publicly available datasets.

Protein Crystallization Segmentation and Classification Using Subordinate Color Channel in Fluorescence Microscopy Images.

The accuracy of detecting protein crystals for fluorescence microscopy images is very critical for high throughput and automated systems. Although the trace fluorescent labeling method could highlight protein crystals, reflection and emission from the fluorescence dye is not always due to crystal regions. Therefore, the analysis of the peak wavelength in the emission spectra of a fluorophore may not always yield effective results.

Schema Matching and Data Integration with Consistent Naming on Protein Crystallization Screens.

The data representation as well as naming conventions used in commercial screen files by different companies make the automated analysis of crystallization experiments difficult and time-consuming. In order to reduce the human effort required to deal with this problem, we present an approach for computationally matching elements of two schemas using linguistic schema matching methods and then transform the input screen format to another format with naming defined by the user. This approach is tested on a number of commercial screens from different companies and the results of the experiments showed an overall accuracy of 97 percent on schema matching which is significantly better than the other two matchers we tested.

Tropical Cyclone Intensity Estimation Using a Deep Convolutional Neural Network.

Tropical cyclone intensity estimation is a challenging task as it required domain knowledge while extracting features, significant pre-processing, various sets of parameters obtained from satellites, and human intervention for analysis. The inconsistency of results, significant pre-processing of data, complexity of the problem domain, and problems on generalizability are some of the issues related to intensity estimation. In this study, we design a deep convolutional neural network architecture for categorizing hurricanes based on intensity using graphics processing unit.

Feature analysis for classification of trace fluorescent labeled protein crystallization images.

Background: Large number of features are extracted from protein crystallization trial images to improve the accuracy of classifiers for predicting the presence of crystals or phases of the crystallization process. The excessive number of features and computationally intensive image processing methods to extract these features make utilization of automated classification tools on stand-alone computing systems inconvenient due to the required time to complete the classification tasks. Combinations of image feature sets, feature reduction and classification techniques for crystallization images benefiting from trace fluorescence labeling are investigated.

FocusALL: Focal Stacking of Microscopic Images Using Modified Harris Corner Response Measure.

Automated image analysis of microscopic images such as protein crystallization images and cellular images is one of the important research areas. If objects in a scene appear at different depths with respect to the camera's focal point, objects outside the depth of field usually appear blurred. Therefore, scientists capture a collection of images with different depths of field.

Super-Thresholding: Supervised Thresholding of Protein Crystal Images.

In general, a single thresholding technique is developed or enhanced to separate foreground objects from background for a domain of images. This idea may not generate satisfactory results for all images in a dataset, since different images may require different types of thresholding methods for proper binarization or segmentation. To overcome this limitation, in this study, we propose a novel approach called "super-thresholding" that utilizes a supervised classifier to decide an appropriate thresholding method for a specific image.

Optimizing Associative Experimental Design for Protein Crystallization Screening.

The goal of protein crystallization screening is the determination of the main factors of importance to crystallizing the protein under investigation. One of the major issues about determining these factors is that screening is often expanded to many hundreds or thousands of conditions to maximize combinatorial chemical space coverage for maximizing the chances of a successful (crystalline) outcome. In this paper, we propose an experimental design method called "Associative Experimental Design (AED)" and an optimization method includes eliminating prohibited combinations and prioritizing reagents based on AED analysis of results from protein crystallization experiments.

CrystPro: Spatiotemporal Analysis of Protein Crystallization Images.

Thousands of experiments corresponding to different combinations of conditions are set up to determine the relevant conditions for successful protein crystallization. In recent years, high throughput robotic set-ups have been developed to automate the protein crystallization experiments, and imaging techniques are used to monitor the crystallization progress. Images are collected multiple times during the course of an experiment.

Evaluation of Normalization and PCA on the Performance of Classifiers for Protein Crystallization Images.

In this paper, we investigate the performance of classification of protein crystallization images captured during protein crystal growth process. We group protein crystallization images into 3 categories: noncrystals, likely leads (conditions that may yield formation of crystals) and crystals. In this research, we only consider the subcategories of noncrystal and likely leads protein crystallization images separately.

Evaluation of Semi-supervised Learning for Classification of Protein Crystallization Imagery.

In this paper, we investigate the performance of two wrapper methods for semi-supervised learning algorithms for classification of protein crystallization images with limited labeled images. Firstly, we evaluate the performance of semi-supervised approach using self-training with naïve Bayesian (NB) and sequential minimum optimization (SMO) as the base classifiers. The confidence values returned by these classifiers are used to select high confident predictions to be used for self-training.

Autofocusing for Microscopic Images using Harris Corner Response Measure.

One of the difficulties for proper imaging in microscopic image analysis is defocusing. Microscopic images such as cellular images, protein images, etc. need properly focused image for image analysis.

Real-Time Protein Crystallization Image Acquisition and Classification System.

In this paper, we describe the design and implementation of a stand-alone real-time system for protein crystallization image acquisition and classification with a goal to assist crystallographers in scoring crystallization trials. In-house assembled fluorescence microscopy system is built for image acquisition. The images are classified into three categories as non-crystals, likely leads, and crystals.