Long intergenic non-coding RNAs modulate proximal protein-coding gene expression and tolerance to Candidatus Liberibacter spp. in potatoes.

Long intergenic non-coding RNAs (lincRNAs) are emerging as regulators of protein-coding genes (PCGs) in many plant and animal developmental processes and stress responses. In this study, we characterize the genome-wide lincRNAs in potatoes responsive to a vascular bacterial disease presumably caused by Candidatus Liberibacter solanacearum (CLso). Approximately 4397 lincRNAs were detected in healthy and infected potato plants at various stages of zebra chip (ZC) disease progression.

Spatial chemistry of citrus reveals molecules bactericidal to Candidatus Liberibacter asiaticus.

Huanglongbing (HLB), associated with the psyllid-vectored phloem-limited bacterium, Candidatus Liberibacter asiaticus (CLas), is a disease threat to all citrus production worldwide. Currently, there are no sustainable curative or prophylactic treatments available. In this study, we utilized mass spectrometry (MS)-based metabolomics in combination with 3D molecular mapping to visualize complex chemistries within plant tissues to explore how these chemistries change in vivo in HLB-infected trees.

Inhibition of a conserved bacterial dual-specificity phosphatase confers plant tolerance to Liberibacter spp.

"Candidatus Liberibacter spp." are insect-vectored, fastidious, and vascular-limited phytopathogens. They are the presumptive causal agents of potato zebra chip, tomato vein clearing, and the devastating citrus greening disease worldwide.

Evaluation of Liberibacter Asiaticus Efflux Pump Inhibition by Antimicrobial Peptides.

Citrus greening, also known as Huanglongbing (HLB), is caused by the unculturable bacterium Liberibacter spp. (e.g.

Protective effect of and against carbon tetrachloride-induced hepatotoxicity in Wistar rats.

Objectives: The present study aims to investigate the protective effect of and extracts on antioxidant activity and analysis on hepatic marker enzyme levels and histopathological changes in the liver of carbon tetrachloride (CCl) induced hepatotoxicity rats.

Materials And Methods: This study includes 42 adult male Albino Wistar rats randomly divided into seven treatment groups, including control (basal diet, G1), CCl-induced single dose (1.5 ml/kg, i.

Applications of CRISPR/Cas13-Based RNA Editing in Plants.

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system is widely used as a genome-editing tool in various organisms, including plants, to elucidate the fundamental understanding of gene function, disease diagnostics, and crop improvement. Among the CRISPR/Cas systems, Cas9 is one of the widely used nucleases for DNA modifications, but manipulation of RNA at the post-transcriptional level is limited. The recently identified type VI CRISPR/Cas systems provide a platform for precise RNA manipulation without permanent changes to the genome.

Identification and Characterization of Potato Zebra Chip Resistance Among Wild Species.

Potato zebra chip (ZC) disease, associated with the uncultured phloem-limited bacterium, Liberibacter solanacearum (Lso), is transmitted by the potato psyllid . Potato ZC disease poses a significant threat to potato production worldwide. Current management practices mainly rely on the control of the psyllid to limit the spread of Lso.

Cardio-protective effects of leaves and fruit extract in doxorubicin-induced cardiomyopathy in rats.

Background: The cardio-protective effects of and are well-recognized in Ayurveda for its antimicrobial, antidiabetic and antioxidant potentials. The present study evaluates the effects of leaves (Tct.LE) and fruits (Tce.

A BTB-TAZ protein is required for gene activation by Cauliflower mosaic virus 35S multimerized enhancers.

The Arabidopsis (Arabidopsis thaliana) BTB-TAZ DOMAIN PROTEIN 2 (BT2) contains an N-terminal BTB domain, a central TAZ zinc-finger protein-protein interaction domain, and a C-terminal calmodulin-binding domain. We previously demonstrated that BT2 regulates telomerase activity and mediates multiple responses to nutrients, hormones, and abiotic stresses in Arabidopsis. Here, we describe the essential role of BT2 in activation of genes by multimerized Cauliflower mosaic virus 35S (35S) enhancers.

Potato Zebra Chip: An Overview of the Disease, Control Strategies, and Prospects.

Potato ( L.) is an important food crop worldwide. As the demand for fresh and processed potato products is increasing globally, there is a need to manage and control devastating diseases such as zebra chip (ZC).

Plant hairy roots enable high throughput identification of antimicrobials against Candidatus Liberibacter spp.

A major bottleneck in identifying therapies to control citrus greening and other devastating plant diseases caused by fastidious pathogens is our inability to culture the pathogens in defined media or axenic cultures. As such, conventional approaches for antimicrobial evaluation (genetic or chemical) rely on time-consuming, low-throughput and inherently variable whole-plant assays. Here, we report that plant hairy roots support the growth of fastidious pathogens like Candidatus Liberibacter spp.

Hepatoprotective effects of on mitochondrial enzymes and inflammatory markers in carbon tetrachloride-induced hepatotoxicity.

Objective: The present investigation was aimed to evaluate the hepatoprotective potential of ethanolic extract of () and its isolated quercetin fraction on carbon tetrachloride (CCl)-induced hepatotoxicity.

Materials And Methods: The rats were divided into 6 groups and each group contained 6 rats. CCl (1.

Phytotherapeutic efficacy of the medicinal plant L.

Diabetes is a chronic, lifelong condition due to inadequate production of insulin or the cells does not properly respond it. Recently, the significance and effectiveness of herbal drugs associated with diabetes has emerged. The aim of the present study was to determine the anti-diabetic effects of L.

Contributions of Particle Image Velocimetry to Helicopter Aerodynamics: A Review.

The advancement of flow measurement techniques continues to extend experimental boundaries and thus significantly contributes to improving our understanding of both basic and applied aerodynamics. This is particularly apparent in the case of particle image velocimetry (PIV), where its application has furthered the existing knowledge in several areas of helicopter rotor aerodynamics. The complex nature of helicopter rotor flows presents unique challenges to experimentalists, including transonic flow, concentrated vortices and dynamic stall.

Implant surgical guides: From the past to the present.

Advent of osseointegration has rapidly led to use of dental implants over recent years. Implant complications are often inadvertent sequelae of improper diagnosis, treatment planning, surgical method, and placement. This can be overcome by using surgical guides for implant positioning.

Evaluation of the inclination of maxillary occlusal plane on mounted casts using a fixed value and customized nasion indicator in artex articulator: Facebow system - An in-vivo comparative study.

Aim: To evaluate and compare the variations in the inclination of occlusal plane of casts mounted on Artex articulator using a facebow with a fixed value and customized nasion indicator.

Materials And Methods: Twenty two subjects were selected for this investigation. Two maxillary impressions were made, and casts poured.