Part II: Impedance-based DNA biosensor for detection of isolated strains of phytopathogen Ralstonia solanacearum.

In Part I, we demonstrated the complete development of a label-free, ultra-low sample volume requiring DNA-based biosensor to detect Ralstonia solanacearum, an aerobic non-spore-forming, Gram-negative, plant pathogenic bacterium, using non-faradaic electrochemical impedance spectroscopy (nf-EIS). We also presented the sensor's sensitivity, specificity, and electrochemical stability. In this article, we highlight the specificity study of the developed DNA-based impedimetric biosensor to detect various strains of R.

Part I: Non-faradaic electrochemical impedance-based DNA biosensor for detecting phytopathogen - Ralstonia solanacearum.

Herein, we report for the first time the development of a label-free, non-faradaic, and highly sensitive DNA-based impedimetric sensor using micro-sized gold interdigitated electrodes (IDE) to detect a soil-borne agricultural pathogen Ralstonia solanacearum. A universal 30 oligomer single-stranded DNA (ssDNA) probe lpxC4 having specificity towards R. solanacearum is successfully immobilized on the surface of IDE along with mercaptohexanol.

Predicting the invasion risk of rugose spiraling whitefly, Aleurodicus rugioperculatus, in India based on CMIP6 projections by MaxEnt.

Background: Rugose spiraling whitefly (RSW), Aleurodicus rugioperculatus Martin, is a highly polyphagous invasive pest native to Central America. The occurrence of A. rugioperculatus in the Oriental region was first reported from Pollachi, Tamil Nadu, India in 2017.

Corrigendum: Characterization of Spp. Inciting Vascular Wilt of Tomato and Its Management by a -Based Biocontrol Consortium.

[This corrects the article DOI: 10.3389/fpls.2021.

Characterization of Spp. Inciting Vascular Wilt of Tomato and Its Management by a -Based Biocontrol Consortium.

Though the vascular wilt of tomato caused by the species of is globally reported to be a complex disease in certain countries, for example, India, our studies indicated that the disease is caused by either f. spp. (Fol) or (FS) with the Fol being widely prevalent.

The involvement of the Type Six Secretion System (T6SS) in the virulence of on brinjal.

is an important soil-borne plant pathogen which causes bacterial wilt in a large number of crops. Bacterial Type Six Secretion System (T6SS) is known to participate in pathogenesis, bacterial interaction and inter-bacterial competition. Contribution of T6SS in the virulence of on eggplant ( L) is studied.

Development of T3SS Mutants ( and of , Evaluation of Virulence Attenuation in Brinjal and Tomato-A Pre-requisite to Validate T3Es of .

is an important plant pathogen which infects a large number of agriculturally important crops. The Type Three Secretion System (T3SS) plays a major role in its pathogenicity by secreting type III effectors (T3Es) which overthrow the host defence mechanism. The secretion of T3Es is transcriptionally regulated by and its secretion is dependent on the pili formed by gene.

Identification of virulence factors and type III effectors of phylotype I, Indian Ralstonia solanacearum strains Rs-09-161 and Rs-10-244.

Ralstonia solanacearum is a well-known phytopathogen causing bacterial wilt in a large number of agriculturally important crops. The pathogenicity of R. solanacearum is expressed due to the presence of various virulence factors and effector proteins.

Diversity, biocontrol, and plant growth promoting abilities of xylem residing bacteria from solanaceous crops.

Eggplant (Solanum melongena L.) is one of the solanaceous crops of economic and cultural importance and is widely cultivated in the state of Goa, India. Eggplant cultivation is severely affected by bacterial wilt caused by Ralstonia solanacearum that colonizes the xylem tissue.

Genome Sequencing of Ralstonia solanacearum Biovar 3, Phylotype I, Strains Rs-09-161 and Rs-10-244, Isolated from Eggplant and Chili in India.

Ralstonia solanacearum Indian strains Rs-09-161 and Rs-10-244 were isolated from the coastal region of Goa and from the Andaman Islands. We report the draft genome sequences of these representative isolates infecting solanaceous vegetables in India.

Antecedent caloric intake and glucose excursion following a subsequent meal in Type 1 diabetes.

Background: In the present study, we tested the hypothesis that calories consumed at a prior meal (lunch) may impair glycemic control after a subsequent meal (supper) even if the pre-supper glucose did not differ regardless of the size of the lunch meal.

Methods: Nine subjects with Type 1 diabetes using continuous subcutaneous (s.c.