The genus consists of purple nonsulfur photosynthetic alphaproteobacteria known for their diverse metabolic capabilities. Here, we report the genome sequence and initial characterization of a novel species, strain M37P, isolated from Mushroom hot spring runoff in Yellowstone National Park at 37 °C. Genome-based analyses and initial growth characteristics helped to define the differentiating characteristics of this species and identified it as an aerobic anoxygenic phototroph (AAP).
View Article and Find Full Text PDFMicrobiol Resour Announc
October 2019
The genus has only one species characterized microbiologically and two genomes sequenced. We have sequenced the genome of a unique strain isolated from Yellowstone National Park and found it to be a distinct new species. is suggested to be a member of the aerobic anoxygenic photosynthetic bacteria.
View Article and Find Full Text PDFThe family is often represented by its thermophilic and radiotolerant species. and have been extensively studied, contributing to defining the characteristics of the family. We have now sequenced the genome of a strain, isolated from Yellowstone National Park, to further characterize the family.
View Article and Find Full Text PDFThe draft genome sequence of the moderately thermophilic bacterium Schleiferia thermophila strain Yellowstone (Bacteroidetes), isolated from Octopus Spring (Yellowstone National Park, WY, USA) was sequenced and comprises 2,617,694 bp in 35 contigs. The draft genome is predicted to encode 2,457 protein coding genes and 37 tRNA encoding genes and two rRNA operons.
View Article and Find Full Text PDFHere we present the genome of strain Exiguobacterium sp. AT1b, a thermophilic member of the genus Exiguobacterium whose representatives were isolated from various environments along a thermal and physicochemical gradient. This genome was sequenced to be a comparative resource for the study of thermal adaptation with a psychroactive representative of the genus, Exiguobacterium sibiricum strain 255-15, that was previously sequenced by the U.
View Article and Find Full Text PDFStreptavidin is an extracellular tetrameric protein produced by Streptomyces avidinii. A series of hybrid gene fusions consisting of Bacillus signal peptide coding regions fused to the mature streptavidin sequence were constructed. B.
View Article and Find Full Text PDFHistidine decarboxylase, the synthetic enzyme for histamine, was partially purified from regions of rat or rabbit brain rich in the enzyme. The enzyme was purified using ion exchange and hydrophobic column chromatography and chromatofocusing. Approximately 70-fold and 110-fold enrichments were attained from rat and rabbit brain, respectively.
View Article and Find Full Text PDFSoutheast Asian J Trop Med Public Health
December 1988
Leptospira interrogans serovar canicola strain moulton was grown to a high cell density in a protein-free medium with hemin. The hemolysin produced in this culture system showed a greatly expanded spectrum of hemolysis as compared to previous reports of leptospiral hemolysin produced in a more traditional culture system containing serum.
View Article and Find Full Text PDFSoutheast Asian J Trop Med Public Health
June 1988
Leptospira interrogans serovar canicola strain moulton was grown to a high cell density in a protein-free medium. When hemin was added to this medium, hemolysin was produced. Hemolysin was not detected when other porphyrins or cytochrome C were substituted for hemin in the medium.
View Article and Find Full Text PDFAn improved and simplified purification procedure has been developed for the isolation of the Bacillus subtilis glucose dehydrogenase which has resulted in a 10 fold higher yield of pure enzyme. The purification procedure utilizes gene cloning and an additional ammonium sulfate step to facilitate the removal of contaminating proteins. The procedure requires fewer chromatographic steps than previously reported, thus simplifying the procedure.
View Article and Find Full Text PDFThe DNA sequence of the structural gene for glucose dehydrogenase (EC 1.1.1.
View Article and Find Full Text PDFAt physiological concentrations, zinc stimulates the activity of pyridoxal kinase, enhancing the formation of pyridoxal phosphate, which in turn enhances the activity of glutamic acid decarboxylase. In toxic doses, zinc inhibits glutamic acid decarboxylase directly and may adversely influence the GABA receptor sites. Zinc-binding proteins, which are inducible by zinc, have been identified in brain and regulate the steady state concentration of free Zn2+.
View Article and Find Full Text PDFJ Biol Chem
October 1983
Bacillus subtilis glucose dehydrogenase (EC 1.1.1.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
February 1983
Glucose dehydrogenase of Bacillus subtilis is a developmental enzyme that is not found in growing (vegetative) cells but is synthesized after the differentiation process that leads to the production of endospores has started. We have isolated the gene coding for this enzyme from a lambda Charon 4A phage library of B. subtilis DNA.
View Article and Find Full Text PDFInositol 2-dehydrogenase (EC 1.1.1.
View Article and Find Full Text PDFLate during sporulation, Bacillus subtilis produces glucose dehydrogenase (GlcDH; EC 1.1.1.
View Article and Find Full Text PDFThe types and distribution of obligate thermophilic bacteria were found to be similar in a thermal gradient resulting from man-made thermal pollution and the thermal gradients of two natural hot springs located in Colorado.
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