Publications by authors named "Raleigh Slyman"
Article Synopsis
- Collective migration of epithelial tissues is vital for developmental processes and maintaining tissue structure, requiring precise coordination of motion influenced by the substrate's mechanical properties.
- The study finds that epithelial monolayers migrate faster on viscoelastic substrates with slower stress relaxation, leading to less deformation and enhanced fluidity.
- Conversely, on faster-relaxing substrates, monolayers face more resistance, resulting in slower migration and limited cell movement, highlighting the importance of substrate characteristics in cell dynamics.
Double-Barrel Perfusion System for Modification of Luminal Contents of Intestinal Organoids.
Methods Mol Biol
February 2024
Organoids are 3D cultures of self-organized adult or pluripotent stem cells with an epithelial membrane enclosing a defined fluid-filled lumen. These organoids have been demonstrated with a wide range of organotypic tissue types, but the enclosed nature of the structure restricts access to the lumen and apical surface of the cell membrane. To increase the potential applications of organoids, new technologies are required to provide access to the lumen of the organoid and apical surface of the epithelial cell membrane to enable new biomedical studies.
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- Breast cancer becomes invasive when carcinoma cells break through the basement membrane (BM), a barrier that separates the tumor from surrounding tissue.
- Researchers created a 3D model to study how multiple cancer cells invade the BM collectively, finding that they use a combination of proteases and mechanical forces without relying on invadopodia.
- The study reveals that the invasion process involves both the expansion of cell volume, which stretches the BM, and local forces that help breach it, highlighting a key mechanism in cancer metastasis.
Intestinal organoids are 3D cell structures that replicate some aspects of organ function and are organized with a polarized epithelium facing a central lumen. To enable more applications, new technologies are needed to access the luminal cavity and apical cell surface of organoids. We developed a perfusion system utilizing a double-barrel glass capillary with a pressure-based pump to access and modify the luminal contents of a human intestinal organoid for extended periods of time while applying cyclic cellular strain.
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