[Role of intrarenal product TNF-alpha in the development of glomerular and tubulointerstitial tissues changes in elderly patients with diabetic nephropathy].

The aim of this study was to investigate the expression of TNF-alpha produced in the kidney in patients with diabetic nephropathy (DN) type 2 diabetes and its impact on the development of glomerular and tubulointerstitial tissue changes in these patients. The survey was conducted in 49 elderly patients (mean age 66.53 +/- 3.

[The role of IL-6 in the development of morphological changes in renal tissue in elderly patients with type 2 diabetes complicated by diabetic nephropathy].

Studies in recent years suggest a role for inflammation, proinflammatory cytokines in particular, the development of microvascular complications of diabetes, including nephropathy. We investigated the expression of IL-6 in the biopsy tissue kidneys (in glomeruli and interstitium) in 24 elderly patients with type 2 diabetes complicated by diabetic nephropathy. The significant influence of intrarenal expression of IL-6 on the development of morphological (glomerular and tubulointerstitial) changes in renal tissue in patients with diabetic nephropathy is shown.

[Effect of expression of IL-6 cytokines in renal tissue on clinical and morphological picture of diseases in patients over 60 years with IgA-nephropathies].

In this paper we analyzed the expression of cytokine IL-6 in biopsy tissue of the kidneys in IgA-nephropathy patients in different age groups, its relationship with clinical and morphological picture of the disease. The study showed that IL-6 affects the formation of histopathological changes in kidney tissue and does not depend on the age of the patient. Given that the duration of the disease affects the expression of IL-6 expression in glomeruli (regardless of age of the patient) the role of IL-6 in the progression of the disease is confirmed.

[Role of intrarenal mononuclear cells apoptosis in the mechanisms of pathogenesis IgA-nephropathy in patients with different age groups].

Apoptosis plays an important role in the mechanism of regulation of the development of glomerulonephritis. We explored the role of apoptosis of mononuclear cells in patients with intrarenal IgA-nephropathy in different age groups and its relation to clinical laboratory, the morphological pattern of the disease. The position of the acceleration of the process of apoptosis intrarenal mononuclear cells in the elderly was confirmed.

[The role of infection in IgA-nephropathy development in patients of different age groups].

Currently, much attention is given to studying the etiological role of bacterial and viral infection in the development and progression of IgA-nephropathy. We analyzed the renal biopsy tissue from 117 patients with IgA-nephropathy in presence of an infectious antigen. We have discovered that the presence of an infectious antigen does not depend on the age of a patient.

[Age-related features of the mesangial proliferative glomerulonephritis].

This article presents the data of 118 patients (77 men and 41 women) with mesangial proliferative glomerulonephritis, aged 22-70 years. It is concluded that the rate of progression and severity of chronic glomerulonephritis, even within the same morphological form, is determined by age.

[New approaches to therapy in glomerulonephritis patients].

Before starting treatment of chronic glomerulonephritis it is desirable to determine the patient's drug sensitivity in vitro because in the absence of such sensitivity chemotherapy should be preceded by immunomodulation with tactivin or thymalin and 3-5 sessions of plasmapheresis to stimulate drug sensitivity. Further measures include prednisolone or cyclophosphamide intravenously for 3-5 days in a dose 300-500 followed by oral administration. Active treatment lasts for 4-8 weeks.

[New findings in the pathogenesis of chronic glomerulonephritis].

30 untreated patients with membranoproliferative glomerulonephritis (MPGN) with isolated urinary syndrome were examined for cellular and humoral immunity. There was a significant (p < 0.05) reduction of the number of T-helpers/inductors (T4/4B4) and early undifferentiated lymphoid precursors with the marker enzyme.

[Kinetics of lymphoid cell subpopulations in the mouse spleen in the early period after unilateral nephrectomy].

The content of total T- and B-lymphocytes, T-rosette-forming at 37 degrees, T-autorosette-forming (ARF), FcIgM+, FcIgG+ and TDT+ lymphocytes is determined in mice after unilateral nephrectomy. The number of ARF, FcIgM+ and TDT+ lymphocytes increased 17 and 48 hours after the nephrectomy. The coincidence in time of the lymphocyte population increase with acquisition of morphogenetic activity by the spleen lymphoid cells allows one to assume their participation in the regenerative proliferation of renal epithelium.

[Subpopulations of peripheral blood T-lymphocytes in patients with chronic glomerulonephritis].

Patients with membranous proliferative glomerulonephritis in the presence of nephrotic syndrome of without it were investigated for subpopulation composition of T lymphocytes and their functional activity in pHA blast transformation test. Though no difference was revealed in E+- and Fc+-receptor occurrence, both spontaneous and PHA-induced malformation of autoreactive T cells were observed. The results justified the previous data on the deficiency in lymphopoiesis of undifferentiated cells with end deoxynucleotidyltransferase as a marker.

[Mechanisms of the development of chronic interstitial nephritis].

Certain developmental mechanisms of chronic interstitial nephritis are discussed. The organism's sensitivity to various medicinal agents is supposedly of importance. Determination of the IgE content has been suggested as a diagnostic test.

[Changes in primordial lymphoid organs in chronic glomerulonephritis].

Changes in primordial lymphoid organs (bone marrow and thymus) with respect to the content of a serum thymic factor in the blood and specific activity of end desoxynucleotidyltransferase in the bone marrow and peripheral blood were studied in patients with chronic glomerulonephritis with and without the nephrotic syndrome. The mechanisms of autoimmunization were also studied with respect to the presence of renal tissue autoantigen antibodies. Alteration of primordial lymphoid organs was not attended by the autoimmune changes, i.

[Immunologic aspects of the pathogenesis of chronic glomerulonephritis].

In untreated patients suffering from membranoproliferative glomerulonephritis, membranous glomerulonephritis and focal glomerulosclerosis with and without the nephrotic syndrome, no convincing evidence in favour of the autoimmune nature of chronic glomerulonephritis could be obtained, since the patients showed the low frequency of the demonstration of antibodies to tissue antigens and the lack of lymphocytes sensitization to the patient's autoantigen. Moreover, there were no convincing data on the impairment of the subpopulation composition of mature T lymphocytes. The patients with chronic glomerulonephritis manifested the deficiency of the production of the early post-thymic immunoregulatory cells.

[Nephrotic syndrome (clinico-morphological, immunological and biochemical aspects)].

Morphological, immunological and biochemical aspects of pathogenesis and manifestation of the nephrotic syndrome in patients with chronic glomerulonephritis were considered on the basis of the authors' result and literature data. High clinical importance of the lymphocyte stimulation index for patients' follow-up was emphasized. 131I-albumin metabolism was studied in 105 patients phenotyping of nephrotic hyperlipoproteinemia was done in 28.

[Role of terminal deoxyribonucleotidyl transferase in stimulating lymphocyte DNA synthesis as affected by PHA].

3H-thymidine incorporation was measured in DNA of the human lymphocytes from the peripheral blood after its PHA-stimulation in vitro. The increased PHA-stimulation was established after addition to the medium of exogenous terminal deoxynucleotidyl transferase (TDT) for cultures with small level of PHA-stimulation only. TDT without PHA exerted no stimulating action on the culture of lymphocytes.