Preclinical evaluation of Ga-labeled folic acid conjugates for visualization of inflammatory foci.

Introduction: Folate receptors (FR) have been considered a convenient target for different radiopharmaceuticals in recent years. Multifarious Ga-labeled folate conjugates have been proposed as promising agents for the PET imaging of FR-overexpressing malignant neoplasms. In addition, radiolabeled folate-based conjugates can be effective for imaging non-tumor pathological foci characterized by a pronounced cluster of activated macrophages.

Improvement of End-of-Synthesis Radiochemical Purity of Lu-DOTA-PSMA-Ligands with Alternative Synthesis Approaches: Conversion Upswing and Side-Products Minimization.

Background: Radiochemical purity is a key criterion for the quality of radiopharmaceuticals used in clinical practice. The joint improvement of analytical methods capable of identifying related radiochemical impurities and determining the actual radiochemical purity, as well as the improvement of synthesis methods to minimize the formation of possible radiochemical impurities, is integral to the implementation of high-tech nuclear medicine procedures. PSMA-targeted radionuclide therapy with lutetium-177 has emerged as an effective treatment option for prostate cancer, and [Lu]Lu-PSMA-617 and [Lu]Lu-PSMA have achieved global recognition as viable radiopharmaceuticals.

Computational Research of the Efficiency of Using a Three-Layer Panel Made of Highly Porous Polystyrene Concrete.

This paper presents linking computational research of the multilayer structure of the cladding of a three-layer panel made of highly porous polystyrene concrete developed using a new technology in comparison with traditional ones. The calculation of the thermal efficiency of the exterior fence was carried out in three stages, where the thermal regime was calculated from the values of temperature fields in the ELCUT 6.6 system, and the humidity and air modes were determined by the analytical method in the Maple system.

Enhancing Automated Brain Tumor Detection Accuracy Using Artificial Intelligence Approaches for Healthcare Environments.

Medical imaging and deep learning models are essential to the early identification and diagnosis of brain cancers, facilitating timely intervention and improving patient outcomes. This research paper investigates the integration of YOLOv5, a state-of-the-art object detection framework, with non-local neural networks (NLNNs) to improve brain tumor detection's robustness and accuracy. This study begins by curating a comprehensive dataset comprising brain MRI scans from various sources.

Radiolysis-Associated Decrease in Radiochemical Purity of Lu-Radiopharmaceuticals and Comparison of the Effectiveness of Selected Quenchers against This Process.

The radiolytic degradation of vector molecules is a major factor affecting the shelf life of therapeutic radiopharmaceuticals. The development of time-stable dosage forms of radiopharmaceuticals is the key to their successful implementation in clinical practice. Using [Lu]Lu-PSMA-617 molecule as an example, the time dependence of the change in radiochemical purity (RCP, %) under radiolysis conditions was studied.

Improved Feature Parameter Extraction from Speech Signals Using Machine Learning Algorithm.

Speech recognition refers to the capability of software or hardware to receive a speech signal, identify the speaker's features in the speech signal, and recognize the speaker thereafter. In general, the speech recognition process involves three main steps: acoustic processing, feature extraction, and classification/recognition. The purpose of feature extraction is to illustrate a speech signal using a predetermined number of signal components.

Pharmacokinetic Properties of Ga-labelled Folic Acid Conjugates: Improvement Using HEHE Tag.

The folate receptor (FR) is a promising cell membrane-associated target for molecular imaging and radionuclide therapy of cancer (FR-α) and potentially also inflammatory diseases (FR-β) through use of folic acid-based radioconjugate. FR is often overexpressed by cells of epithelial tumors, including tumors of ovary, cervix, endometrium, lungs, kidneys, etc. In healthy tissues, FR can be found in small numbers by the epithelial cells, mainly in the kidneys.

[Purification of extracellular proteinases from B. subtilis SKB 256 by biospecific chromatography].

Abstract-A simple and efficient method of preparing highly purified extracellular proteinases of B. subtilis B-1 (SKB 256) has been developed. A sorbent based on sorsilen impregnated with hemoglobin or cytochrome c has been synthesized for this purpose.

[Enzymatic conversion of invertase substrates in a water-organic medium].

The behavior of intact and immobilized invertase in aqueous and water-organic media has been studied. In a water-organic medium, the transferase properties of the enzyme changed: pH optima of intact and immobilized invertase undergo shifts of 0.5 units: the temperature optimum decreases (50 degrees C and 25 degrees C in aqueous and water-organic media, respectively).

[Isolation of myoinositol from solutions of enzymatic biotransformation].

The transferase reaction between phospholipids and inositol catalyzed by phospholipase D on phase interface in water-organic solvent systems was studied. Optimal conditions for phosphatidylinositol synthesis in water-organic solvent heterogeneous system were determined. The rapid separation of the hydrophobic components, phospholipids, from water-soluble products, alcohols, was observed in the systems with organic solvents.

[Anti-inflammatory activity of domestic papain].

The influence of domestic papain to the course of experimental inflammation due to formalin, dextrane, histamine, serotonin and infectious arthritis has been studied. The domestic papain in doses of 0.325 and 0.

[Pharmacological study of papain from the papaya plant cultivated in Uzbekistan].

It was experimentally established that papain from papaya cultivated in Uzbekistan possesses a pronounced proteolytic activity: 0.1, 0.5, and 1% papain solutions decreased the weight of burn crust in vitro and accelerated experimental burn healing in vivo.

[Immunoenzyme assay of staphylococcal toxin using polyamide micropore membranes].

The optimum conditions for extraction of catalytic active conjugates of alpha-amylase and antibodies for staphylococcal toxin (ST) have been created. The optimum correlation of antibodies and enzyme for the effective use of the extracted conjugate during enzyme immunoassay for ST has been established. The covalent immobilization of antibodies on the micropore polyamide membranes has been carried out.

[Change in phospholipid composition of synovial fluid in children with various types of arthritis].

Phospholipid composition of synovial fluid was studied in patients with reactive, rheumatoid and juvenile chronic forms of arthritis as compared with that of normal synovial fluid. The most pronounced alterations of the synovial fluid phospholipid composition (additional phospholipid fractions, increase in content of lyso-derivatives) were found in the patients with rheumatoid and juvenile chronic arthritis, which appear to occur due to activation of endogenous phospholipases A2, C and lysophospholipase A1.

[Various lipases for producing products enriched with polyenic acids in fish fat hydrolysis].

The enzymic hydrolysis of fish with lipases from various sources was studied. The lipase from the fungus Rhizopus microsporus preferentially removes saturated fatty acids, while lipase from the pyloric caeca of salmon unsaturated fatty acids upon hydrolysis of fish fats. The enzymes can be used to obtain fatty products enriched with eicosanopentaenoic acid, mono- and diacylglycerols by enzymic hydrolysis of the ivasi fat.

[Adsorption immobilization of alkaline lipase].

A polyamide with the covalently coupled phosphatidyl ethanolamine was used for affinity adsorption of an alkaline lipase from Pseudomonas aeruginosa. The immobilization resulted in increase of the enzyme specific activity. Some properties of native and adsorbed enzyme were compared.

[Regional lymphotropic therapy].

The article deals with the results of experimental studies on 10 male and female dogs conducted under intrapleural hexenal anaesthesia. The rate of elimination of the lymphotrophic dye indigo carmine from the subserous depot in the wall of the gallbladder, stomach, and small intestine under normal conditions and in regional stimulation of lymphatic drainage was studied. The rate of total elimination increased 2.

[Affinity chromatography for purification of phospholipase A2 from the venom of Central Asian cobra].

A method of affinity chromatography was developed for purification of phospholipase A2(PL-A2) from the Central Asian cobra venon. The enzyme was covalently coupled to a polyamide sorbent with phosphatidilethanolamine (PEA) and cytotoxin (CT). The effect of CA2+ concentration and the ion strength of the solution on the enzyme adsorption was studied.

[Purification of phospholipase C isoenzyme 1 from Clostridium perfringens and its properties].

A procedure for the purification of isoenzyme I of phospholipase C from Cl. perfringens was developed. The isoenzyme was purified to homogeneity (data from disc electrophoresis) using affinity chromatography on polycephamide and gel filtration through Ultrogel AcA-54, the enzyme yield being 41%.

[Properties of phospholipase D from rat liver mitochondria].

The properties of membrane-bound mitochondrial phospholipase D were investigated. The enzyme was shown to catalyze the hydrolysis of endogenous mitochondrial phospholipids, particularly phosphatidylethanolamine. The phospholipase activity was maximal at pH 5.

[Changes in the phospholipid composition of mitochondria during heat incubation].

Incubation of liver mitochondria at 37 degrees C causes changes in the phospholipid composition, such as the decrease in the levels of major phospholipids (e.g. phosphatidylcholine, phosphatidylethanolamine, cardiolipin) and their lysoderivatives as well as an increase in the levels of phosphatidic and lysophosphatidic acids.

[Lysophospholipase activity in mitochondrial membranes of the rat liver].

Changes in the amount of phospholipids and lysophospholipids of mitochondria and their fragments have been studied under long-term heat incubation. A discrepancy is found between a decrease in the content of phospholipids as a result of their hydrolysis by mitochondrial phospholipase A2 and accumulation of the corresponding lysoderivatives. Data are presented which show that all this is a result of lysoderivatives splitting by lysophospholipase A.

[Immobilization of phospholipase A2 from Central Asian cobra venom on polyamide sorbents].

The effect of the immobilization technique and the ligand nature on catalytic properties of phospholipase A2 from the cobra venom was studied. Preparations of phospholipase A2 adsorbed on and covalently bound to polyamide sorbents were obtained. The enzyme was coupled to polyamide beads modified with glutaraldehyde.