Surface plasmon resonance based indirect immunoassay for detection of 17β-estradiol.

A surface plasmon resonance (SPR) based immunosensor is presented for highly sensitive and selective detection of 17β-estradiol by the indirect competitive inhibition immuno assay, employing anti-17 β-estradiol antibody as high molecular weight (HMW) interactant. Immobilization of estradiol-BSA conjugate onto the nano thin gold surface was accomplished by covalent amide linkage through self assembled monolayer. The proposed biosensor is simple to fabricate, reproducible and exhibit excellent sensitivity for estrogen (detection limit,1 pg mL) without any significant interference from structurally similar steroidal hormone, progesterone and non-steroidal compound bisphenol-A.

Surface plasmon resonance based immunosensor for serological diagnosis of dengue virus infection.

Surface plasmon resonance (SPR) is a promising tool in sensor technology for biomedical applications. An SPR based immunosensor was established for label free and real time assay for the serological diagnosis of dengue virus infection employing the dengue virus antigen as the sensing element. The dengue virus antigen conjugated with bovine serum albumin is covalently immobilized on a gold sensor chip via activated self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid, by amide coupling.