Fenugreek hydrogel-agarose composite entrapped gold nanoparticles for acetylcholinesterase based biosensor for carbamates detection.

A biosensor was fabricated to detect pesticides in food samples. Acetylcholinesterase was immobilized in a novel fenugreek hydrogel-agarose matrix with gold nanoparticles. Transparent thin films with superior mechanical strength and stability were obtained with 2% fenugreek hydrogel and 2% agarose.

Invertase-nanogold clusters decorated plant membranes for fluorescence-based sucrose sensor.

In the present study, invertase-mediated nanogold clusters were synthesized on onion membranes, and their application for sucrose biosensor fabrication was investigated. Transmission electron microscopy revealed free nanoparticles of various sizes (diameter ~5 to 50 nm) along with clusters of nanogold (~95 to 200 nm) on the surface of inner epidermal membranes of onions (Allium cepa L.).

Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.

Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation. For this purpose, the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads and Ch-GG-AgNPs-GOD (glucose oxidase) beads were prepared. The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.

1,3-β-Glucanase from Vigna aconitifolia and its possible use in enzyme bioreactor fabrication.

Endo-1,3(4)-β-glucanase (EC 3.2.1.

Electrochemical β(1→3)-d-glucan biosensors fabricated by immobilization of enzymes with gold nanoparticles on platinum electrode.

β(1→3)-d-Glucan sensors were fabricated using bi-enzyme and tri-enzyme immobilized systems with gold nanoparticles (GNPs) to increase sensitivity. The plant β(1→3)-D-glucanase (βG), glucose oxidase (GOD) or/and peroxidase (POD) in agarose-corn flour-gelatin (ACG) matrix were coated on platinum disc electrode to detect soluble β(1→3)-D-glucan. The atomic force microscopy (AFM) revealed that GNPs embedded in ACG formed tiny islands/clusters with enzymes.

Fabrication of photometric dip-strip test systems for detection of beta(1-->3)-D-glucan using crude beta(1-->3)-D-glucanase from sprouts of Vigna aconitifolia.

Efforts have been made to fabricate enzyme dip-strip test systems for detecting beta(1-->3)-D-glucan. Beta(1-->3)-D-glucanase from sprouts of Vigna aconitifolia (commonly known as moth bean, 8-day old) with high specific activity (244 U mg(-1)) was co-entrapped with glucose oxidase (GOD) in different combinations of composite polymer matrices of agarose (A), gelatin (G), polyvinyl alcohol (PVA) and corn flour (CF). The enzyme immobilized membranes were checked for immobilization yield, pH and temperature optima, swelling index, thermal, operational and storage stability, and morphology by scanning electron microscopy.

Characterization of alpha-mannosidase from Erythrina indica seeds and influence of endogenous lectin on its activity.

alpha-mannosidase from Erythrina indica seeds is a Zn(2+) dependent glycoprotein with 8.6% carbohydrate. The enzyme has a temperature optimum of 50 degrees C and energy of activation calculated from Arrhenius plot was found to be 23 kJ mol(-1).